GB/T 46451-2025Determination of chiral amino acids — High performance liquid chromatography tandem mass spectrometry with pre-column derivatization (English PDF)

Foreword

This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1: Structure and Drafting Rules of Standardization Documents". Drafting.

Please note that some content in this document may involve patents. The publishing organization of this document assumes no responsibility for identifying patents.

This document was proposed and is under the jurisdiction of the National Technical Committee on Standardization of Biochemical Testing (SAC/TC387).

This document was drafted by: Shenzhen Institute of Metrology and Quality Inspection, Shenzhen Inspection Group Medical Testing Laboratory, and China Testing Technology Research Institute.

Institute, Shenzhen Institute for Drug Control (Shenzhen Medical Device Testing Center), Shenzhen Agricultural Product Quality and Safety Inspection and Testing Center, Hebei Province Food Inspection and Research Institute, Shanghai Chemical Industry Research Institute Co., Ltd., China Certification & Inspection Group Hunan Co., Ltd., Lanzhou Baiyuan Gene Technology Co., Ltd.

The company, the China Metrology and Testing Society, and the Yibin Municipal Inspection and Testing Center.

The main drafters of this document are: Yang Guowu, Li Ziying, Ji Yuan, Sun Liping, Tang Shujun, Liu Chengwen, Wang Jinmei, Jin Yibao, Luo Min, and Kuang Huiying.

Zhang Yuxin, Zhou Lihua, Wang Yujie, Zhang Yan, Shi Guohua, Lei Wen, Yu Jinglan, Qin Meirong, Li Meifang, Liu Yao, Wang Ping, Wang Qi, Jin Long, Su Jiating Huang Chengyi, Zhu Zechao, Chen Xin, Zhao Yunxia, Xiao Weimin, Liu Xiangrong, Yang Yangzhongfu, Che Tuanjie, Liu Han, Zheng Xiaoling.

High-efficiency pre-column derivatization for the determination of chiral amino acids Liquid chromatography-tandem mass spectrometry

1 Scope

This document describes the use of pre-column derivatization high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for the detection of D-/L-alanine, D-/L-serine, and D-/L-proline.

Acids, D-/L-valine, D-/L-threonine, D-/L-cysteine, D-/L-leucine, D-/L-isoleucine, D-/L-asparagine, D-/L- Aspartic acid, D-/L-glutamine, D-/L-glutamic acid, D-/L-histidine, D-/L-phenylalanine, D-/L-arginine, D-/L-tryptophan D-/L-L-Lysine, D-/L-Ornithine, D-/L-Citriline, D-/L-Hydroxyproline, D-/L-alpha-Aminoadipic acid, D-/L-Phosphosphireine Methods for determining D-/L-kynurenine, D-/L-2-aminobutyric acid, D-/L-methionine and D-/L-tyrosine.

This document applies to the determination of the content of the above-mentioned free chiral amino acids in biochemical reagents and biological samples.

2 Normative references

GB/T 6682

3 Terms and Definitions

The following terms and definitions apply to this document.

4 Principles

After the protein in the sample was precipitated with sulfosalicylic acid, the amino acids were extracted using formic acid aqueous solution, and then reacted with triethylamine under the catalysis of...

N-alpha-(5-fluoro-2,4-dinitrophenyl)-L-propanamide underwent a derivatization reaction, and the derivative was analyzed by triple quadrupole liquid chromatography-tandem mass spectrometry.

Quantitative analysis using the internal standard method.

5 Reagents and Consumables

Unless otherwise stated, all reagents used in this method are of analytical grade, and the water is Grade I water as specified in GB/T 6682.