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GB/T 46144-2025Chemicals — Fish cell line acute toxicity — The RTgill-W1 cell line assay (English PDF)

化学品　鱼类细胞系急性毒性　虹鳟鳃细胞系（RTgill-W1）试验

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

August 29, 2025

Implementation date

December 1, 2025

Scope

GB/T 46144-2025 is the English-translated version of 化学品　鱼类细胞系急性毒性　虹鳟鳃细胞系（RTgill-W1）试验.

GB/T 46144-2025 is the Chinese national standard covering acute fish toxicity measured on gill cells in a dish instead of on live fish — the RTgill-W1 line and the three viability endpoints, the exposure in a sealed plate that stops volatile substances escaping, and the correlation back to the fish LC50. It exists to replace the acute fish test, which kills animals for a number this assay can often give. First edition, in force from 1 December 2025. Issued on 29 August 2025, it has been in force since 1 December 2025.

Document preview — GB/T 46144-2025

National Standard of the People's Republic of China

ICS: 13.300
Classification: A 80

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

PrefaceIII
1 Scope1
2 Normative references1
3 Terms and definitions, abbreviations1
4 Test Principle2
5 Test substance information2
6 Reference3
7 Experimental Preparation3
8 Test Procedure3
9 Data Analysis and Results Evaluation9
10 Quality Assurance and Quality Control9
11 Results Report10
Appendix A (Normative) Preparation of test media and reagents11
Appendix B (Informative) Passaging and Cryopreservation of RTgil-W1 Cells13
Reference16

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1: Structure and drafting rules for standardization documents" Drafting.

Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.

This document is proposed and coordinated by the National Technical Committee for Standardization of Hazardous Chemicals Management (SAC/TC251).

This document was drafted by: Institute of Microbiology, Guangdong Academy of Sciences (Guangdong Microbiological Analysis and Testing Center), Solid Waste Management Department of the Ministry of Ecology and Environment Together with the Chemical Management Technology Center, Shanghai Testing Center, Nanjing Institute of Environmental Sciences of the Ministry of Ecology and Environment, and Pearl River Delta of the Chinese Academy of Fishery Sciences Fisheries Research Institute, China Inspection and Testing Technology (Tianjin) Co., Ltd., and China Chemical Economic and Technological Development Center.

The main drafters of this document are: Mei Chengfang, Xu Yujie, Xu Meiying, Dou Congcong, Wang Lei, Wang Qing, Teng Xiaoming, Wang Peng, Liu Chunxin, Chen Xiaoqian, Lin Muqing, Wang Yingying, Yu Mingxuan, Wang Xiaobing, Xu Lihong, Yang Jing, Deng Tongchu, Zhong Zhen, Guo Jiafeng, Hao Changfu, and Tian Shengjiang.

Chemicals Fish Cell Line Acute Toxicity Rainbow Trout Gill Cell line (RTgil-W1) assay

1 Scope

This document describes the test method for the acute toxicity of chemicals to fish cell lines using the rainbow trout gill cell line (RTgil-W1).

This document is applicable to the testing and evaluation of the acute toxicity of chemicals to fish cell lines.

2 Normative references

GB/T 21852

GB/T 21853

GB/T 22052

GB/T 22228

GB/T 22229

GB/T 27860

GB/T 42426

3 Terms, definitions, and abbreviations

3.1 Terms and Definitions

The following terms and definitions apply to this document.

3.1.1 Cell viability

The percentage of cells that are in a healthy state (i.e., metabolically active, with intact cell membranes and organelles).

3.1.2 effect concentration;ECx

The concentration of test substance that causes a change of x% in cell viability compared to the negative control or solvent control during a given test period.

3.1.3

Leibovitz L-15 medium is supplemented with 5% fetal bovine serum and optional antibiotics.

Note. Commonly used antibiotics include 0.5% gentamicin.

3.1.4 L-15/ex medium

Protein-free medium containing the same salts, galactose, and pyruvate as Leibovitz L-15 medium.

Note. Used for cell exposure experiments.

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 20 pages — is available in the English PDF.

Referenced standards

Normative references

GB/T 21853 · GB/T 22052 · GB/T 22228 · GB/T 22229 · GB/T 27860

Editions of GB/T 46144

Edition	Title	Revision	Status
GB/T 46144-2025	Chemicals - Fish cell line acute toxicity - The RTgill-W1 cell line assay	current edition	Current

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