GB/T 45588-2025Detection and identification of Potyvirus zeananus (English PDF)

玉米矮花叶病毒检疫鉴定方法

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

April 25, 2025

Implementation date

November 1, 2025

Scope

GB/T 45588-2025 is the English-translated version of 玉米矮花叶病毒检疫鉴定方法.

GB/T 45588-2025 is the Chinese national standard covering finding maize dwarf mosaic virus in maize seed and plant tissue — the taxonomic placement and the background information in the annex, the quarantine sampling, the serological detection, the molecular RT-PCR detection, the reagents and equipment the laboratory needs, and the combination of results that decides the identification, since a mosaic symptom on its own does not separate this virus from the others that mottle maize. First edition, in force from 1 November 2025. Issued on 25 April 2025, it has been in force since 1 November 2025.

Document preview — GB/T 45588-2025

National Standard of the People's Republic of China

ICS: 65.020.20
Classification: B 16

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Maize Dwarf Mosaic Virus Classification Information
5 Principles of the method
6 Reagents, instruments, equipment and utensils
6.1 Reagents

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1: Structure and drafting rules for standardization documents" Drafting.

Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.

This document is proposed and coordinated by the National Technical Committee on Plant Quarantine Standardization (SAC/TC271).

This document was drafted by: China Inspection and Quarantine Scientific Research Institute, Fujian Academy of Agricultural Sciences Fruit Research Institute, and Fuzhou Customs Technical Center.

The main drafters of this document are: Zhang Yongjiang, Xie Lixue, Shen Jianguo, Chen Xihong, Zhao Zhenxing and Dong Zheng.

Quarantine and Identification Methods for Maize Dwarf Mosaic Virus

1 Scope

This document describes serological and molecular methods for the detection of Maize Dwarf Mosaic Virus.

This document applies to the quarantine identification of maize dwarf mosaic virus.

2 Normative references

GB/T 6682

3 Terms and definitions

There are no terms or definitions that require definition in this document.

4 Maize Dwarf Mosaic Virus Classification Information

Chinese name. Maize dwarf mosaic virus.

Scientific name. Potyviruszeananus. Abbreviation. MDMV.

Classification. Patatavirales, Potyviridae, Genus Poty- virus).

Additional information on Maize Dwarf Mosaic Virus is provided in Appendix A.

5 Principles of the method

The serological characteristics and genomic characteristics of maize dwarf mosaic virus are the basis for quarantine identification of the virus.

Method. Through the effective combination of these methods, it is determined whether the sample contains corn dwarf mosaic virus.

6 Reagents, instruments, equipment and utensils

6.1 Reagents

Unless otherwise specified, all reagents were of analytical grade.

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 17 pages — is available in the English PDF.

Referenced standards

Normative references

GB/T 6682Water for analytical laboratory use - Specification and test methods

Editions of GB/T 45588

Edition	Title	Revision	Status
GB/T 45588-2025	Detection and identification of Potyvirus zeananus	current edition	Current

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