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GB/T 45587-2025Detection and identification of Potyvirus glycitessellati (English PDF)

大豆花叶病毒检疫鉴定方法

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

April 25, 2025

Implementation date

November 1, 2025

Scope

GB/T 45587-2025 is the English-translated version of 大豆花叶病毒检疫鉴定方法.

GB/T 45587-2025 is the Chinese national standard covering finding soybean mosaic virus in seed and plant material — the taxonomic placement and the background information in the annex, the sampling drawn under the plant quarantine sampling method, the serological detection, the RT-PCR that reads the genome directly, the reagents and the equipment, and the combination of methods that has to agree before a lot is declared infected. First edition, in force from 1 November 2025. Issued on 25 April 2025, it has been in force since 1 November 2025.

Document preview — GB/T 45587-2025

National Standard of the People's Republic of China

ICS
65.020.20
Classification
B 16

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Contents

  • Foreword
  • 1 Scope
  • 2 Normative references
  • 3 Terms and definitions
  • 4 Soybean mosaic virus classification information
  • 5 Principles of the method
  • 6 Reagents, instruments, equipment and utensils
  • 6.1 Reagents

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1: Structure and drafting rules for standardization documents" Drafting.

Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.

This document is proposed and coordinated by the National Technical Committee on Plant Quarantine Standardization (SAC/TC271).

This document was drafted by: China Institute of Inspection and Quarantine, Fuzhou Customs Technical Center, and Huai'an Hongze District Agricultural Cadre School.

The main drafters of this document are: Zhang Yongjiang, Chen Xihong, Jin Ling, Shen Jianguo, Zhao Zhenxing and Dong Zheng.

Quarantine and Identification Methods for Soybean Mosaic Virus

1 Scope

This document describes serological and molecular methods for the detection of soybean mosaic virus.

This document applies to the quarantine identification of soybean mosaic virus.

2 Normative references

GB/T 6682

3 Terms and definitions

There are no terms or definitions that require definition in this document.

4 Soybean mosaic virus classification information

Chinese name. soybean mosaic virus.

English name. soybean mosaic virus. Abbreviation. SMV.

Classification. Patatavirales, Potyviridae, Genus Poty- virus).

Additional information on soybean mosaic virus is provided in Appendix A.

5 Principles of the method

The serological characteristics and genomic features of soybean mosaic virus are the basis for quarantine identification of the virus.

Optical RT-PCR detection method; through the effective combination of these methods, it is determined whether the sample carries soybean mosaic virus.

6 Reagents, instruments, equipment and utensils

6.1 Reagents

Unless otherwise specified, all reagents were of analytical grade.

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 17 pages — is available in the English PDF.

Referenced standards

Editions of GB/T 45587

EditionTitleRevisionStatus
GB/T 45587-2025Detection and identification of Potyvirus glycitessellaticurrent editionCurrent

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