GB/T 45243-2025Determination of thiamine, riboflavin, pyridoxine, niacin, niacinamide and caffeine in health foods (English PDF)

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1: Structure and drafting rules for standardization documents" Drafting.

This document specifies the technical requirements related to food quality. For food safety requirements, please refer to relevant laws, regulations, policies and food safety standards. document.

This document replaces GB/T 5009.197-2003 "Thiamin hydrochloride, pyridoxine hydrochloride, niacin, niacinamide and caffeine in health foods" Compared with GB/T 5009.197-2003, in addition to structural adjustments and editorial changes, the main technical changes are as follows.

a) The scope of application has been changed (see Chapter 1, Chapter 1 of the 2003 edition);

b) Added the determination of riboflavin (see 5.3.2, 5.4.2, 7.4, 7.5, Chapter 8 and Appendix A);

c) The sample preparation and processing methods have been changed (see 7.1, 7.2, 5.1 of the 2003 edition);

d) The reference conditions for liquid chromatography have been changed (see 7.3, 5.2 of the 2003 edition);

e) The detection limit and quantification limit have been changed (see Chapter 10, Chapter 1 of the 2003 edition).

Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.

This document was proposed and coordinated by the National Technical Committee for Standardization of Special Foods (SAC/TC466).

This document was drafted by: Jiangzhong Pharmaceutical Co., Ltd., Hangzhou Center for Disease Control and Prevention (Hangzhou Health Supervision Institute), Jiangsu Ai Lande Nutrition Products Co., Ltd., China Light Industry Technology Innovation Center Co., Ltd., Zhejiang Xinweishi Biotechnology Co., Ltd., Biostime (China) Co., Ltd.

Co., Ltd., China Food Fermentation Industry Research Institute Co., Ltd., Jiangsu Huashan Health Technology Co., Ltd., Dongpeng Beverage (Group) Co., Ltd., Amway (China) Daily Products Co., Ltd., Zhejiang Yangshengtang Natural Medicine Research Institute Co., Ltd., SGS-CSTC Standards Technical Services Co., Ltd., Thomson By-Health Co., Ltd. and Hebei Chenguang Testing Technology Service Co., Ltd.

The main drafters of this document are: Chen Fang, Jin Quan, Xu Xiaoming, Wu Zhuying, Zheng Minmin, Yan Jun, Zhong Qiding, An Hongmei, Li Yong, Chi Huazhong, Wang Daobing, Hu Hai'e, Peng Xianwu, Huang Zhiming, Pan Minyao, Luo Shihui, Zhang Xiaofang, Xu Haoran, Liu Yang, Chang Jun, Xu Jun, Zhong Shunhao, Zhang Peixia, Wu Xiangqian, Li Xueli, Liu Chunli, Wang Xiaodong, Yang Lufang, Huang Zhenghua, He Ruikun, Wang Wenchang, Yue Hongwei.

The previous versions of this document and the documents it replaces are as follows.

— First published in 2003 as GB/T 5009.197-2003;

— This is the first revision.

Thiamine, riboflavin, pyridoxine, niacin, Determination of Nicotinamide and Caffeine

1 Scope

This document describes the HPLC determination of thiamine, riboflavin, pyridoxine, niacin, niacinamide and caffeine in health foods. method.

This document applies to thiamine, Determination of riboflavin, pyridoxine, niacin, niacinamide and caffeine.

2 Normative references

GB/T 6682

3 Terms and definitions

There are no terms or definitions that require definition in this document.

4 Principle

The sample was extracted and diluted with 20% methanol-0.1% phosphoric acid solution, separated by high performance liquid chromatography column and detected by ultraviolet detector to retain Qualitative analysis was performed by time and quantitative analysis was performed by external standard method.

5 Reagents or materials

Unless otherwise specified, only chromatographic grade reagents were used.