GB/T 44739-2024Determination of Lycium barbarum polysaccharides in wolfberry and interrelated product—Ion chromatography method (English PDF)

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1: Structure and drafting rules for standardization documents" Drafting.

Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.

This document is proposed and coordinated by the China National Institute of Standardization.

This document was drafted by: Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Ningxia Hui Autonomous Region Institute for Drug Control, Qingdao Municipal Resources Chemistry and New Materials Research Center, China National Institute of Standardization, Gansu Pharmaceutical Group Science and Technology Innovation Research Institute Co., Ltd., Rizhao Quality Inspection Testing Institute, Lycium barbarum Science Research Institute of Ningxia Academy of Agricultural and Forestry Sciences, Lanzhou Food and Drug Inspection and Testing Institute, Beijing Forestry University, Northwest Normal University Fan University, Beijing Academy of Science and Technology Analysis and Testing Institute (Beijing Physical and Chemical Analysis and Testing Center), Gansu University of Chinese Medicine, Inner Mongolia Autonomous Region Product Quality Inspection Institute, Swiss Metrohm China Co., Ltd., Qingdao Shenghan Chromatography Technology Co., Ltd., Ningxia Zhongqi Ecological Agriculture Technology Co., Ltd., Laiyang Inspection and Testing Center, Guangdong Yifang Pharmaceutical Co., Ltd., By-Health Co., Ltd., Infinitus (China) Co., Ltd., Zhejiang Tiancao Biotechnology Co., Ltd., Perfect (Guangdong) Daily Necessities Co., Ltd., Nanjing Zhongke Pharmaceutical Co., Ltd., Suzhou University.

The main drafters of this document are: Wang Ningli, Di Duolong, Pei Dong, Ma Ling, Zhang Lan, Huang Xinyi, Xi Xingjun, Lan Tao, Zhao Xi, Liu Jianfei, Qiu Guoyu, Zhang Xiyan, Lei Jiandu, Zang Yani, Li Yan, Yu Xiaoyi, Yang Yingli, Ma Zongwei, Ping Jiwen, Wang Wei, Le Shengfeng, Wang Han, Wang Xiaofang, Liu Xiaoxiao, Meng Lingling, Zhou Xuan, Guo Mei, Gao Yang, Li You, Tian Haifeng, Jia Zhankui, Chen Xiangdong, Li Zhenyu, Sun Hongmei, Liu Weide, Wu Junchao, Zhou Yajie, Feng Peng, Li Duxin, Wang Yong, Zhang Yusheng, and Tan Qi.

Determination of Lycium barbarum Polysaccharides in Lycium barbarum and Its Products Ion chromatography

1 Scope

This document describes the ion chromatography method for the determination of Lycium barbarum polysaccharides in Lycium barbarum and its product, Lycium barbarum puree.

This document is applicable to the determination of Lycium barbarum polysaccharide content in Lycium barbarum mature fruits of various varieties and Lycium barbarum products made through drying and processing.

The determination of the content of Lycium barbarum polysaccharides in other Lycium barbarum products can also refer to this method.

2 Normative references

GB/T 6682

3 Terms and Definitions

The following terms and definitions apply to this document.

4 Principles

The sample was purified by ethanol and extracted by water reflux to obtain Lycium barbarum polysaccharide, which was then digested with trifluoroacetic acid and the product was separated by anion chromatography column.

The content of Lycium barbarum polysaccharides in the sample was calculated based on the peak area of each monosaccharide by chromatographic determination and external standard method.

5 Reagents and Materials

Unless otherwise specified, only analytical grade reagents were used.

5.1 Water. GB/T 6682, Grade 1.

5.2 Galactose (C6H12O6, CAS No.. 59-23-4). purity >= 98%.

5.3 Arabinose (C5H10O5, CAS No.. 5328-37-0). purity >= 98%.

5.4 Glucose (C6H12O6, CAS No.. 50-99-7). purity >= 98%.

5.5 Rhamnose (C6H14O6, CAS No.. 10030-85-0). purity >= 98%.

5.6 Xylose (C5H10O5, CAS No.. 58-86-6). purity >=98%.

5.7 Mannose (C6H12O6, CAS No.. 3458-28-4). purity >= 98%.

5.8 Galacturonic acid (C6H10O7, CAS No.. 14982-50-4). purity >= 98%.