GB/T 44620-2024Determination of phloridzin in apple and apple products—High performance liquid chromatography method (English PDF)

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1: Structure and drafting rules for standardization documents" Drafting.

Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.

This document is proposed and coordinated by the China National Institute of Standardization.

This document was drafted by: Beijing University of Chemical Technology, China National Institute of Standardization, Jinan Fruit Research Institute of China National Federation of Supply and Marketing Cooperatives, China Agricultural University, Shenzhen Yirui Biotechnology Co., Ltd., Zhejiang Tiancao Biotechnology Co., Ltd., Hebei Guanzhuo Testing Technology Co., Ltd.

Co., Ltd., Beijing Academy of Science and Technology Analysis and Testing Institute (Beijing Physical and Chemical Analysis and Testing Center), Hefei Normal University, Shandong Tianyuan Yingkang Testing and Evaluation Technology Co., Ltd., Hebei Chenguang Testing Technology Service Co., Ltd., Zhongshan Hongli Healthy Food Industry Research Institute Ltd., Food and Drug Environmental Inspection Institute (Shandong) Group Co., Ltd., Liheweidao (Qingdao) Food Industry Co., Ltd., Nanjing Zhongke Pharmaceutical Industry Co., Ltd.

The main drafters of this document are: Wei Yun, Lu Yanzhen, Xi Xingjun, Lan Tao, Yan Yiyong, Zheng Xiaodong, Ni Hanwen, Zhou Wenfeng, Wei Tongyu, Cao Ning, Lu Runhua, Liu Yuanyuan, Liu Cong, Jin Lan, Zeng Jiajia, Yu Yingchun, Wang Wenjuan, Wang Wei, He Tianyu, Yan Chen, Shao Yundong, Huang Mengqiao, Hins Cheung, Zhang Yuchi, Zhai Cuicui, Lei Minzhi, Zhang Xiaofang, Jiao Liwei, Sun Xuewen, Huang Wenwen, Ju Xiaomin, Li Panpan, Zhao Xingli, Zhongli Jianmin, Li Guosheng, Zhang Yongze, Zhang Mengying, Guan Yuwei, Feng Peng, Zhang Yongchang, Zhou Yajie, Mo Qiuhua, Duan Jiaxin.

Detection method of phlorizin in apple and apple products High performance liquid chromatography

1 Scope

This document describes a method for the determination of phlorizin in apples and apple products using HPLC.

This document is applicable to the determination of phlorizin in apples and apple products.

2 Normative references

GB/T 6379.2

GB/T 6682

3 Terms and definitions

There are no terms or definitions that require definition in this document.

4 Principle

The samples were extracted with ethanol and water (80%), purified with C18 adsorbent, separated by liquid chromatography, and detected by UV detector or diode array.

The samples were detected by the external standard method, and the qualitative analysis was based on the retention time.

5 Reagents and Materials

Unless otherwise specified, only analytical grade reagents were used.

5.1 Water. GB/T 6682, Grade 1.

5.2 Ascorbic acid (C6H8O6, CAS No.. 50-81-7).

5.3 Acetonitrile (C2H3N, CAS No.. 75-05-8). chromatographically pure.

5.4 Ethanol aqueous solution (80%). Use a measuring cylinder to measure 80 mL of ethanol (C2H6O, CAS No.. 64-17-5) and 20 mL of water into a reagent bottle. Shake well.

5.5 Trifluoroacetic acid aqueous solution (0.01%). Measure 1000 mL of water into the reagent bottle with a measuring cylinder, and use a pipette to draw 0.1 mL of chromatographically pure trifluoroacetic acid.

Add fluoroacetic acid (C2HF3O2, CAS No.. 76-05-1) into the reagent bottle and shake well.

5.6 Standard substance. Phloridzin (C21H24O10, CAS. 60-81-1), content >= 98.0%.

5.7 Standard stock solution (1.0 mg/mL). Accurately weigh 25 mg (accurate to 0 0001 g) of phlorizin standard into a 25 mL volumetric flask.

Dissolve in acetonitrile (5.3) and dilute to the mark, shake well, and the product is obtained. Store in a refrigerator at 0°C~4°C away from light. The shelf life is 30 days.