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GB/T 44480-2024Chemicals—Eremias argus acute oral toxicity test (English PDF)

化学品 丽斑麻蜥急性经口毒性试验

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

September 29, 2024

Implementation date

April 1, 2025

Scope

GB/T 44480-2024 is the English-translated version of 化学品 丽斑麻蜥急性经口毒性试验.

GB/T 44480-2024 is the Chinese national standard covering the acute oral toxicity of a chemical to Eremias argus, a lizard used as a terrestrial test animal — the single gavage dose given at graded concentrations, the sample preparation, the seven-day observation, the calculation of the median lethal dose and of the non-lethal and total lethal doses, the test report, and the annex on breeding the lizard in captivity, without which there is nothing to dose. First edition. In force from 1 April 2025. Issued on 29 September 2024, it has been in force since 1 April 2025.

Document preview — GB/T 44480-2024

National Standard of the People's Republic of China

ICS
13.300
Classification
A 80

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Contents

  • Preface... III 1 Scope1
  • 2 Normative references1
  • 3 Terms and Definitions1
  • 4 Experimental Overview1
  • 5 Test methods2
  • 5.1 Materials and Instruments2
  • 5.2 Sample preparation2
  • 5.3 Test operation2
  • 5.4 Result calculation and presentation3
  • 5.5 Quality Assurance and Quality Control3
  • 6 Test Report3
  • Appendix A (Informative) Conditions and methods for domestication and reproduction of the beautiful spotted lizard A Method4
  • Appendix B (Informative) Solution Preparation and Test Observation Record Sheet7
  • References8

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1: Structure and drafting rules for standardization documents" Drafting is required.

Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.

This document is proposed and coordinated by the National Technical Committee on Hazardous Chemicals Management Standardization (SAC/TC 251).

This document was drafted by: Eco-Environmental Research Center of the Chinese Academy of Sciences, China Inspection and Testing (Tianjin) Co., Ltd., China Institute of Inspection and Quarantine Science.

The main drafters of this document are: Chang Jing, Wang Huili, Wan Bin, Xie Yun, Li Jianzhong, Zhou Lili, Liu Chunyan, and Wang Lili.

Acute oral toxicity test of chemicals on Lizard

1 Scope

This document describes the test method for the acute oral toxicity test of chemicals to Lizard lizards, and specifies the test materials, conditions, operations, Basic requirements for quality control, data processing, test reports, etc.

This document is applicable to testing the acute toxic effects of chemicals on the beautiful spotted lizard.

2 Normative references

GB/T 6682

GB/T 31270.9-2014

3 Terms and definitions

The following terms and definitions apply to this document.

3.1 median lethal dose; LD50

The statistical dose of a toxicant that causes half of the animals to die after a single administration of the test substance to experimental animals.

The mass of the test substance received per unit body weight is expressed.

3.2 Maximum non lethal dose; LD0

The highest toxic dose that does not cause death to experimental animals after a single administration of the test substance to experimental animals.

Body weight is expressed as the mass of the test substance.

3.3 Minimum total lethal dose; LD100

The lowest statistical dose of a toxicant that causes all death of experimental animals after a single administration of the test substance to experimental animals.

The body weight is expressed as the mass of the test substance received.

3.4 acute oral toxicity

Health-damaging effects that occur in experimental animals within a short period of time (7 days) after a single oral administration of a test substance.

3.5 Test substances

A pure substance or mixture composed of various elements.

4 Experimental Overview

A series of test substances with different concentrations were orally infused into the beautiful spotted lizard once. The test period was 7 days, and the test data were recorded every day.

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.

Referenced standards

Editions of GB/T 44480

EditionTitleRevisionStatus
GB/T 44480-2024Chemicals - Eremias argus acute oral toxicity testcurrent editionCurrent

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