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GB/T 44293-2024Image measurement method for numerical aperture of microscopic objective (English PDF)

显微物镜数值孔径图像式测量方法

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

August 23, 2024

Implementation date

March 1, 2025

Scope

GB/T 44293-2024 is the English-translated version of 显微物镜数值孔径图像式测量方法.

GB/T 44293-2024 is the Chinese national standard covering image measurement method for numerical aperture of microscopic objective. Issued on 23 August 2024, it has been in force since 1 March 2025.

Document preview — GB/T 44293-2024

National Standard of the People's Republic of China

ICS: 31.260
Classification: L 50

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

PrefaceIII
1 Scope1
2 Normative references1
3 Terms and Definitions1
4 Measurement principle2
5 Measuring device4
5.1 Device composition4
5.2 Imaging Illumination Module4
5.3 Focusing Module4
5.4 Measuring the lighting module5
5.5 Measurement module5
5.6 Detection Module5
5.7 Data Processing Module5
6 Measurement environment5
7 Measurement steps5
8 Uncertainty Analysis6
Appendix A (Normative) Calculation method of numerical aperture circle diameter and total reflection circle diameter8
Appendix B (Informative) Microscope Objective Numerical Aperture Measurement Results Record Sheet10
Appendix C (Informative) Uncertainty Analysis of Numerical Aperture Measurement of NA1.50 Microscope Objective11
References12

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1: Structure and drafting rules for standardization documents" Drafting is required.

Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.

This document was proposed by the Chinese Academy of Sciences.

This document was prepared by the National Technical Committee on Standardization of Photoelectric Measurement (SAC/TC 487) and the National Technical Committee on Standardization of Optics and Photonics (SAC/TC 103) Jointly managed.

This document was drafted by: Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Qizhiming Optoelectronic Intelligent Technology (Suzhou) Co., Ltd.

Suzhou Guoke Medical Technology Development (Group) Co., Ltd., Suzhou Metrology and Testing Institute, China National Institute of Metrology, Chinese Academy of Sciences Aerospace Information Innovation Research Institute, China University of Metrology, Ningbo Sunny Optical Instruments Co., Ltd., Ningbo Novosun Optics Co., Ltd., Nanjing Zhiqun Optoelectronic Technology Co., Ltd., Zhijiang Laboratory, and Nanjing Schmidt Optical Instrument Co., Ltd.

The main drafters of this document are: Gong Yan, Tang Yuguo, Zhang Yanwei, Lang Song, Li Jun, Xu Yingying, Wang Pu, Zhan Chunlian, Hu Senhu, Cui Zhiying, Yuan Qun, Zhang Jianfeng, Lei Zhen.

Image-based measurement method for numerical aperture of microscope objective

1 Scope

This document describes the measurement principle, measurement device, measurement environment, measurement steps and Uncertainty analysis.

This document applies to the numerical aperture measurement of infinity-corrected microscope objectives with a nominal numerical aperture greater than 1.00.

2 Normative references

GB/T 26600

JJF 1059.1-2012

3 Terms and definitions

The following terms and definitions apply to this document.

3.1 diameter of total reflection circle

The circular shape formed on the image sensor by light reflected from the lower surface of the cover glass and the air interface with an incident angle less than the critical angle of total reflection The diameter of the pattern.

3.2 diameter of numerical aperture circle

The circular shape formed on the image sensor by light reflected from the lower surface of the cover glass and the air interface with an incident angle greater than the critical angle of total reflection The diameter of the pattern.

3.3 Objective

The first component of an imaging system that forms the initial image of an object.

Note. It is composed of lenses, lens assemblies and related parts, and can be used alone or in combination with tube lenses.

[Source. GB/T 27668-2023, 3.1.106, modified]

3.4 Infinity-corrected objective

The initial image distance of the object is corrected at infinity, so an objective lens corresponding to the tube lens is required.

[Source. GB/T 27668-2023, 3.1.106.3, modified]

3.5 numerical aperture; NA

It is expressed as n sinu, where n is the refractive index of the medium between the objective and the object, and u is half the aperture angle of the objective.

[Source. GB/T 27668-2023, 3.1.10.4, modified]

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 17 pages — is available in the English PDF.

Referenced standards

Normative references

GB/T 26600 · JJF 1059.1-2012

Editions of GB/T 44293

Edition	Title	Revision	Status
GB/T 44293-2024	Image measurement method for numerical aperture of microscopic objective	current edition	Current

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