GB/T 43544-2023Oral care and cleaning products—Laboratory test method for inhibition rate of toothpastes to dental calculus (English PDF)

Foreword

This document complies with the provisions of GB/T 1:1-2020 "Standardization Work Guidelines Part 1: Structure and Drafting Rules of Standardization Documents" Drafting:

Please note that some content in this document may be subject to patents: The publisher of this document assumes no responsibility for identifying patents:

This document is proposed by China Light Industry Federation:

This document is under the jurisdiction of the National Oral Care Products Standardization Technical Committee (SAC/TC492):

This document was drafted by: Guangzhou Institute of Quality Supervision and Inspection, Shanghai Meijiajing Daily Chemical Co:, Ltd:, Suzhou Jinmao Daily Chemical Co:, Ltd:

Co:, Ltd:, Guangzhou Shuke Industrial Co:, Ltd:, Jiangxi Chengzhi Daily Chemical Co:, Ltd:, Suzhou Qingxin Health Technology Co:, Ltd:, Heilongjiang Light Industry Science and Technology Co:, Ltd:

Research Institute, Haolai Chemical (Zhongshan) Co:, Ltd:, Chongqing Dengkang Oral Care Products Co:, Ltd:, Yunnan Baiyao Group Health Products Co:, Ltd:

Co:, Ltd:, Hangzhou Namei Zhikang Technology Co:, Ltd:, Liuzhou Liangmianzhen Co:, Ltd:, Infinitus (China) Co:, Ltd:, and Naisi Group have Co:, Ltd:, Suzhou Luye Daily Necessities Co:, Ltd:, Colgate-Palmolive (China) Co:, Ltd:, Guangzhou Zhonghan Oral Products Co:, Ltd:, Huaian Zongheng Biotechnology Co:, Ltd: Huaiyin Branch, Dr: Kang Daily Chemical Group Co:, Ltd:, Zhejiang Aishang Daily Necessities Co:, Ltd:, Guangzhou Qiancai Cosmetics Products Co:, Ltd:, Haoyikang Biotechnology (Guangzhou) Co:, Ltd:, Stander Kechuang Medical Technology (Qingdao) Co:, Ltd:, Guangzhou Star Yuedong Stock Co:, Ltd:, Foshan Nanhai District Heshun Anfu Daily Necessities Co:, Ltd:, Shanghai Lili Daily Necessities Co:, Ltd:, Fujian Aijili Daily Chemical Co:, Ltd:

Company, Shenzhen Xiaokuo Technology Co:, Ltd:, Shanghai Gemang Biotechnology Co:, Ltd:, Yangzhou Beijiajie Daily Chemical Co:, Ltd:, Air Force Medical University School of Dental Medicine:

The main drafters of this document: Jia Fang, Shi Yilei, Chen Jianfen, Chen Minshan, Xu Haiyan, Mao Jianlin, Sun Dongfang, Zheng Wei, He Qiying, Deng Quanfu, Zhang Zhiwei, Fan Yu, Hu Yongzhi, Gao Yan, Peng Yan, Liu Dong, Zheng Zhuoli, Zhong Xiji, Da Chengzhu, Wang Chunmei, Zhao Guosheng, Xu Chunsheng, Huang Xiujuan, Gong Jingrong, Li Lifen, Hu Yin, Yang Xuyi, Chen Wanjin, Yin Kuo, Fang Hao, Lu Liuhua, Li Xinyu, Chen Xiaobin, Li Yiqing, Han Jindou, Li Lu, Zhou Yi, Liu Jiasu, Xie Yu, Zeng Yuyang, Xia Zemin, Tan Jianhua, Xi Shaofeng, Li Gang:

Oral cleaning care product toothpaste inhibits dental calculus Laboratory test methods for rate

1 Scope

This document describes the principles, reagents and materials, instruments and equipment, and measurement steps of the laboratory test method for toothpaste’s inhibition rate on dental calculus: calculate:

This document is applicable to the determination of the inhibition rate of toothpastes on dental calculus that uses inhibition of crystal growth as its mechanism of action:

2 Normative reference documents

GB/T 6682

GB 5009

3 Terms and definitions

There are no terms or definitions to be defined in this document:

4 Principles

When there is thick dental plaque, the calcium and phosphate in the saliva locally accumulate on it and gradually form (hydroxy) calcium phosphate minerals, which are dental plaque:

stone: Under constant pH conditions, using hydroxyapatite powder as a seed crystal, the calcium and phosphorus elements in artificial saliva react on the surface of the seed crystal Generate (hydroxy) calcium phosphate precipitates to simulate the plaque calcification process during the formation of dental calculus: By measuring calcium in artificial saliva concentration, and calculate the inhibition rate of the sample on dental calculus:

5 Reagents and materials

5:1 General requirements: Unless otherwise stated, the reagents used in this method are of analytical grade, and the test water is in accordance with the requirements specified in GB/T 6682: grade water:

5:2 Anhydrous calcium chloride (CaCl2):

5:3 Sodium dihydrogen phosphate (NaH2PO4): 5:4 Sodium chloride (NaCl): 5:5 Sodium hydroxide (NaOH):

5:6 Sodium pyrophosphate (Na4P2O7):

5:7 Hydroxyapatite [Ca5(PO4)3(OH)]: purity >=97%:

5:8 Sodium hydroxide solution (0:5mol/L): Weigh 0:08g (accurate to 0:01g) of sodium hydroxide (5:5), dissolve it in water and transfer to 10mL volumetric flask, dilute to volume and shake well: