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GB/T 42470-2023Nanotechnologies - Assessment of nanomaterial toxicity using dechorionated zebrafish embryo (English PDF)

纳米技术 基于斑马鱼胚胎的纳米材料毒性评价

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Issued by

State Administration for Market Regulation, China National Standardization Administration

Level / Type

National · Recommended

Issue date

March 17, 2023

Implementation date

October 1, 2023

Scope

GB/T 42470-2023 is the English-translated version of 纳米技术 基于斑马鱼胚胎的纳米材料毒性评价.

GB/T 42470-2023 is the Chinese standard "Nanotechnologies - Assessment of nanomaterial toxicity using dechorionated zebrafish embryo".

Its scope clause reads: This document specifies a method for rapid assessment of the toxicity of nanomaterials (early life stages of fish, 0HPF~120HPF), including Detailed protocol for removing egg membranes and a complete protocol for evaluating nanomaterial toxicity using egg membrane-depleted zebrafish embryos: The focus of this document is to test the Toxicity of nanomaterials: This document applies to the toxicity testing of commercial and non-commercial nanomaterials:. Its clauses include terms and definitions.

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Document preview — GB/T 42470-2023

National Standard of the People's Republic of China

ICS
07.120
Classification
C 04

Issued by: State Administration for Market Regulation, China National Standardization Administration

Contents

  • Preface
  • Introduction
  • 1 Scope
  • 2 Normative references
  • 3 Terms and Definitions
  • 4 Abbreviations
  • 5 material
  • 6 equipment
  • 7 Step
  • 8 Test report
  • Appendix A (informative) mechanical membrane rupture method
  • Appendix B (Informative) Zebrafish Embryo Spawning Step
  • Appendix C (Informative) Result Verification

1 Scope

This document specifies a method for rapid assessment of the toxicity of nanomaterials (early life stages of fish, 0HPF~120HPF), including

Detailed protocol for removing egg membranes and a complete protocol for evaluating nanomaterial toxicity using egg membrane-depleted zebrafish embryos: The focus of this document is to test the

Toxicity of nanomaterials:

This document applies to the toxicity testing of commercial and non-commercial nanomaterials:

2 Normative references

The contents of the following documents constitute the essential provisions of this document through normative references in the text: Among them, dated references

For documents, only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document:

GB/T 6682-2008 Analytical laboratory water specifications and test methods

ISO /T S12805 Nanotechnologies-Materialsspecifi-

Note: GB/T 37156-2018 Nanotechnology Material Specification Guidelines for Nano-object Characteristics (ISO /T S12805:2011, IDT)

ISO /T R13014 Guidelines for Characterization of Physicochemical Properties of Nanotechnology Nanomaterials Before Toxicological Evaluation (Nanotechnologies-Guid- ment)

Note: GB/T 39261-2020 Guidelines for Characterization of Physicochemical Properties of Nanotechnology Nanomaterials Before Toxicological Evaluation (ISO /T R13014:2012, IDT)

ISO /T S17200 Nanotechnology powder nanoparticle characterization and measurement (Nanotechnology-Nanoparticlesin

ISO /T R18196 Nanotechnologies-Measurement Matrix for Characterization of Nanoobjects

Note: GB/T 41204-2021 Nanotechnology Nano-object Characterization Measurement Technology Matrix (ISO /T R18196:2016, MOD)

ISO /T S80004-1 Nanotechnology Terminology Part 1: Core Terms (Nanotechnologies-Vocabulary-Part

1: Core terms)

Note: GB/T 30544:1-2014 Nanotechnology Terminology Part 1: Core Terms (ISO /T S80004-1:2010, IDT)

ISO /T S80004-2 Nanotechnology Terminology Part 2: Nanoobjects (Nanotechnologies-Vocabulary-Part

2: Nano-objects)

OECDTG236: Fish Embryo Acute Toxicity Test OECD Guidelines for Testing Chemicals Part 2: OECD Press, Brazil

3 Terms and Definitions

ISO /T S12805, ISO /T R13014, ISO /T S17200, ISO /T S80004-1, ISO /T S80004-2, OECDTG236

GB/T 36388 | YBB 2027 | YBB 6006 | GB/T 45633 | GB/T 42469 | […]

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 30 pages — is available in the English PDF.

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