GB/T 42470-2023Nanotechnologies - Assessment of nanomaterial toxicity using dechorionated zebrafish embryo (English PDF)
纳米技术 基于斑马鱼胚胎的纳米材料毒性评价
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Issued by
State Administration for Market Regulation, China National Standardization Administration
Level / Type
National · Recommended
Issue date
March 17, 2023
Implementation date
October 1, 2023
Scope
GB/T 42470-2023 is the English-translated version of 纳米技术 基于斑马鱼胚胎的纳米材料毒性评价.
GB/T 42470-2023 is the Chinese standard "Nanotechnologies - Assessment of nanomaterial toxicity using dechorionated zebrafish embryo".
Its scope clause reads: This document specifies a method for rapid assessment of the toxicity of nanomaterials (early life stages of fish, 0HPF~120HPF), including Detailed protocol for removing egg membranes and a complete protocol for evaluating nanomaterial toxicity using egg membrane-depleted zebrafish embryos: The focus of this document is to test the Toxicity of nanomaterials: This document applies to the toxicity testing of commercial and non-commercial nanomaterials:. Its clauses include terms and definitions.
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Document preview — GB/T 42470-2023
National Standard of the People's Republic of China
- ICS
- 07.120
- Classification
- C 04
Issued by: State Administration for Market Regulation, China National Standardization Administration
Contents
- Preface
- Introduction
- 1 Scope
- 2 Normative references
- 3 Terms and Definitions
- 4 Abbreviations
- 5 material
- 6 equipment
- 7 Step
- 8 Test report
- Appendix A (informative) mechanical membrane rupture method
- Appendix B (Informative) Zebrafish Embryo Spawning Step
- Appendix C (Informative) Result Verification
1 Scope
This document specifies a method for rapid assessment of the toxicity of nanomaterials (early life stages of fish, 0HPF~120HPF), including
Detailed protocol for removing egg membranes and a complete protocol for evaluating nanomaterial toxicity using egg membrane-depleted zebrafish embryos: The focus of this document is to test the
Toxicity of nanomaterials:
This document applies to the toxicity testing of commercial and non-commercial nanomaterials:
2 Normative references
The contents of the following documents constitute the essential provisions of this document through normative references in the text: Among them, dated references
For documents, only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document:
GB/T 6682-2008 Analytical laboratory water specifications and test methods
ISO /T S12805 Nanotechnologies-Materialsspecifi-
Note: GB/T 37156-2018 Nanotechnology Material Specification Guidelines for Nano-object Characteristics (ISO /T S12805:2011, IDT)
ISO /T R13014 Guidelines for Characterization of Physicochemical Properties of Nanotechnology Nanomaterials Before Toxicological Evaluation (Nanotechnologies-Guid- ment)
Note: GB/T 39261-2020 Guidelines for Characterization of Physicochemical Properties of Nanotechnology Nanomaterials Before Toxicological Evaluation (ISO /T R13014:2012, IDT)
ISO /T S17200 Nanotechnology powder nanoparticle characterization and measurement (Nanotechnology-Nanoparticlesin
ISO /T R18196 Nanotechnologies-Measurement Matrix for Characterization of Nanoobjects
Note: GB/T 41204-2021 Nanotechnology Nano-object Characterization Measurement Technology Matrix (ISO /T R18196:2016, MOD)
ISO /T S80004-1 Nanotechnology Terminology Part 1: Core Terms (Nanotechnologies-Vocabulary-Part
1: Core terms)
Note: GB/T 30544:1-2014 Nanotechnology Terminology Part 1: Core Terms (ISO /T S80004-1:2010, IDT)
ISO /T S80004-2 Nanotechnology Terminology Part 2: Nanoobjects (Nanotechnologies-Vocabulary-Part
2: Nano-objects)
OECDTG236: Fish Embryo Acute Toxicity Test OECD Guidelines for Testing Chemicals Part 2: OECD Press, Brazil
3 Terms and Definitions
ISO /T S12805, ISO /T R13014, ISO /T S17200, ISO /T S80004-1, ISO /T S80004-2, OECDTG236
GB/T 36388 | YBB 2027 | YBB 6006 | GB/T 45633 | GB/T 42469 | […]
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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 30 pages — is available in the English PDF.
Referenced standards
Normative references
- GB/T 6682-2008Water for analytical laboratory use - Specification and test methods
- GB/T 37156-2018Nanotechnologies -- Materials specifications -- Guidance on specifying nano-objects
- GB/T 39261-2020Nanotechnologies. Guidance on physico-chemical characterization of engineered nanoscale materials for toxicologic assessment
- GB/T 41204-2021Nanotechnologies - Measurement technique matrix for the characterization of nano-objects
GB/T 30544
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Related Standards
GB/T 30544.1-2014 — Nanotechnologies -- Vocabulary -- Part 1: Core terms
GB/T 30544.12-2023 — Nanotechnologies - Vocabulary - Part 12: Quantum phenomena in nanotechnology
GB/T 30544.13-2018 — Nanotechnologies -- Vocabulary -- Part 13: Graphene and related two-dimensional (2D) materials
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