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GB/T 40626-2021Detection and identification of Xanthomonas populi ( ex Ridé 1958 ) Ridé &Ridé 1992 (English PDF)

杨树细菌性溃疡病菌检疫鉴定方法

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

October 11, 2021

Implementation date

May 1, 2022

Scope

GB/T 40626-2021 is the English-translated version of 杨树细菌性溃疡病菌检疫鉴定方法.

GB/T 40626-2021 is the Chinese national method for detecting and identifying Xanthomonas populi, the bacterium that causes poplar bacterial canker. It describes symptom inspection, isolation and culture, biochemical characterization and molecular identification of the pathogen.

The identification rests on the disease symptoms, colony morphology, the tobacco hypersensitive reaction, physiological, biochemical and molecular characteristics, and pathogenicity testing, following a set inspection process, while an annex gives background information on the disease.

It applies to quarantine, detection and identification of the bacterium carried in poplar seedlings and other plant material entering or leaving China, and to field surveys and disease monitoring.

Plant quarantine laboratories use it. It was issued on 11 October 2021 and has applied since 1 May 2022.

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Document preview — GB/T 40626-2021

National Standard of the People's Republic of China

ICS
65.020.01
Classification
B 16

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Contents

  • Foreword
  • 1 Scope
  • 2 Normative references
  • 3 Terms and definitions
  • 4 Classification information of germs
  • 5 Principles of the method
  • 6 Inspection process

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules of Standardization Documents" Drafting.

1 Scope

This document describes the methods of symptom inspection, isolation and culture, biochemical characterization and molecular identification of poplar bacterial canker.

This document is applicable to the quarantine, detection and identification of poplar bacterial canker bacteria carried in poplar seedlings and other plant materials in and out of the country, as well as the field Investigation and disease monitoring.

2 Normative references

The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document.

SN/T 1193 Technical requirements for genetic testing laboratories

SN/T 2601 Specification for Routine Testing of Phytopathogenic Bacteria

3 Terms and definitions

There are no terms and definitions that need to be defined in this document.

4 Classification information of germs

Chinese name: Poplar Bacterial Canker

See Appendix A for relevant information about bacterial canker of poplar.

5 Principles of the method

Harmful symptoms, colony morphology, tobacco allergic reactions, physiological and biochemical characteristics, and molecular biological characteristics of poplar bacterial canker And pathogenicity testing is the principle of the quarantine identification method in this document.

6 Inspection process

The detection process is shown in Figure 1.

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.

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