GB/T 40455-2021Detection and identification of Blueberry shock virus (English PDF)

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules of Standardization Documents" Drafting.

1 Scope

This document specifies the quarantine and identification method for Blueberry shock virus.

This document is applicable to the quarantine and identification of blueberries that may carry blueberry shock virus.

2 Normative references

The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document.

GB/T 6682 Analytical laboratory water specifications and test methods

SN/T 2122 Quarantine sampling method for entry and exit plants and plant products

3 Terms and definitions

There are no terms and definitions that need to be defined in this document.

4 Classification information of blueberry shock virus

Chinese name. blueberry shock virus. Scientific name. Blueberryshockvirus, abbreviation. BlShV. Taxonomic status. Bromoviridae, Ilarvirus.

For the host range, disease symptoms, distribution area, transmission route, corpuscle morphology, genome and other information of blueberry shock virus, please refer to the attachment Record A.

5 Principles of the method

The serological and molecular biological characteristics of blueberry shock virus are the main basis for formulating this quarantine identification method. According to BlShV and antibodies The specific reaction between the samples is tested by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and immunochromatographic test strips;

Perform nested RT-PCR, immune capture nested RT-PCR, and real-time fluorescent RT-PCR detection according to the characteristics of the BlShV genome; through these methods Effective combination of the method to determine whether the sample carries BlShV.

6 Instruments, utensils and reagents