GB/T 40175.2-2021Textiles—Methods of biochemical analysis—Part 2: Pyrethroid pesticides (enzyme-linked immunosorbent assay) (English PDF)

1 Scope

This document specifies the enzyme-linked immunosorbent assay method for the detection of residues of seven pyrethroid pesticides, including fenpropathrin, deltamethrin, cypermethrin, beta-cypermethrin, cyfluthrin, RU 38702, and cyphenothrin, in textiles.

This document applies to all kinds of textiles.

2 Normative references

The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. For dated references, only the version corresponding to that date is applicable to this document; for undated references, the latest version (including all amendments) is applicable to this document.

GB/T 6682, Water for analytical laboratory use - Specification and test methods

3 Terms and definitions

There are no terms and definitions that need to be defined in this document.

4 Principle

The pyrethroid pesticides in the sample and the pyrethroid pesticide antigens that have been coated on the microplate in the kit competitively combine with the pyrethroid pesticide antibody; then, add the enzyme-labeled secondary […]

5 Reagents and materials

5.2 Washing liquid: Before use, add water to dilute the concentrated washing solution 5.1h) in a volume ratio of 1:9 (1 part of concentrated washing solution + 9 parts of water).

5.3 Color-developing agent: Mix substrate A solution 5.1e) and substrate B solution 5.1f) in a volume ratio of 1:1 before use.

5.4 N-hexane.

5.5 Petroleum ether.

5.6 Methanol.

5.7 Phosphate buffer solution (0.01 mol/L): Dissolve 8.0 g of sodium chloride, 0.2 g of potassium chloride, 1.44 g of disodium hydrogen phosphate and 0.24 g of potassium dihydrogen phosphate in 800 mL of deionized water; use 0.01 mol/L hydrochloric acid to adjust the pH value of the solution to 7.4; add water to make the volume up to 1 000 mL.

5.8 Methanol-phosphate buffer solution: Mix methanol and buffer solution (5.7) in a volume ratio of 1:4.

6 Equipment and utensils

6.1 Microplate reader: The wavelength is 450 nm.

6.2 Balance: sensitivity of 0.1 g and 0.000 1 g.

6.3 Micro adjustable pipette and matching tips: 50 µL, 100 µL, 1 000 µL.

6.4 Temperature-controllable ultrasonic bath: The working frequency is 40 kHz; the temperature control accuracy is ±5 °C.

6.5 Nitrogen-blowing instrument.

6.6 Oven: The temperature control accuracy is ±1 °C.

6.7 pH meter: It is equipped with glass electrode; the measurement accuracy is at least accurate to 0.1.

7 Test procedure

8 Calculation and expression of results