GB/T 40174-2021Purity detection method of reagent enzymes (English PDF)

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules of Standardization Documents" Drafting.

1 Scope

This document describes the terms and definitions, principles, reagents or materials, equipment, sample preparation, and test procedures of the tool enzyme purity detection method. Steps, result calculation and quality control.

This document is suitable for testing the purity of tool enzymes.

2 Normative references

The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document.

GB/T 6682 Analytical laboratory water specifications and test methods

YY/T 0087 Electrophoresis device

3 Terms and definitions

The following terms and definitions apply to this document.

4 Principle

With the bovine serum albumin standard as a control, the sample was taped after sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis.

Scan the surface, and then calculate the gray value to obtain the quality of the target tool enzyme in the sample, and then use the Folin method to determine the quality of the total protein of the sample Or use the ignition residue inspection method to determine the solid content of the sample to calculate the purity of the tool enzyme.

5 Reagents or materials

Unless otherwise specified, the reagents used are all analytically pure, and the water is secondary water specified in GB/T 6682.