GB/T 36833-2018Detection and identification of Potato virus X (English PDF)

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009.

1 Scope

This standard specifies the quarantine and identification methods for potato X virus.

This standard applies to the quarantine and identification of potato plants, propagation materials and other host plants that may have potato X virus.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this document. For undated references, the latest edition (including all amendments) applies to this document.

GB 7331 Potato seed potato quarantine regulations

SN/T 2122 Quarantine sampling method for inbound and outbound plants and plant products

3 Potato X virus basic information

Chinese name. Potato X virus Scientific name. PotatovirusX Abbreviation. PVX Classification status. Alphaflexiviridae, Potexvirus.

Transmission route. under the natural conditions of the field, the mechanical communication is mainly carried out by the contact friction between different parts of the host plant and the host plants.

In the case of gardening or gardening, it can also be spread by means of tools and tools. Long-distance transmission through the transfer of vegetative propagation materials such as seed potatoes to make. There is information that the potato seed can transmit the virus, but it has little significance in production; no other Solanaceae crops have transmitted the disease.

Poisonous information. According to reports, certain insects such as Melanoplusdifferentialis and Green Talis (Tettigonia) Viridissima's chewing mouthpiece mechanically transmits the virus, Cuscutacampestris and Aphis gossypii (Synchyrtiumendobioticum) can also spread the virus.

See Appendix A for additional information on the Potato X virus.

4 Principle of the method

The genomic characteristics and immunogenicity of PVX are the basis for the quarantine and identification of this virus. Establish reverse transcription based on the genomic characteristics of PVX PCR (RT-PCR), real-time fluorescent reverse transcription PCR (Real-time RT-PCR) and reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay Determination method; establishment of enzyme-linked immunosorbent assay (ELISA) method, immunochromatographic test strip detection method and dot enzyme according to PVX immunogenicity Combined immunosorbent assay (dot-ELISA) method; by an effective combination of these methods, the sample is judged whether it has PVX.

5 Instruments, equipment and reagents