GB/T 36824-2018Detection and identification of Mycosphaerella pini E.Rostrup (English PDF)
松针红斑病菌检疫鉴定方法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
September 17, 2018
Implementation date
April 1, 2019
Scope
GB/T 36824-2018 is the English-translated version of 松针红斑病菌检疫鉴定方法.
GB/T 36824-2018 gives the quarantine and identification method for the pine needle blight fungus Mycosphaerella pini E. Rostrup on pines, other host plants and plant materials.
It applies to seedlings and branches of Pinus and related species. The standard gives basic information on the pathogen, including its scientific name, its many synonyms, its taxonomic position and its asexual form Dothistroma septosporum.
Identification is based on the disease symptoms, the morphological and culture characteristics of the fungus and its molecular biology, with the host and geographical distribution as supporting evidence.
Sampling follows SN/T 2122, and the standard lists the equipment and reagents needed, from microscopes and clean benches to incubators and water baths. It was issued on 17 September 2018 and has applied since 1 April 2019.
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Document preview — GB/T 36824-2018
National Standard of the People's Republic of China
- ICS
- 65.020.01
- Classification
- B 16
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- Foreword
- 1 Scope
- 2 Normative references
- 3 Basic information on pine needle blight
- 4 Principle of the method
- 5 Equipment and reagents
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
1 Scope
This standard specifies the quarantine and identification methods for Mycosphaerella pini in Pinus, other host plants and plant materials.
This standard is applicable to the quarantine and identification of Mycosphaerella pini in the seedlings and branches of Pinus and related species.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this document. For undated references, the latest edition (including all amendments) applies to this document.
SN/T 2122 Quarantine sampling method for inbound and outbound plants and plant products
3 Basic information on pine needle blight
Scientific name: Mycosphaerella pini E. Rostrup apud Munk (Evans, 1984).
Synonyms: Eruptio pini (Rostr.) M.E. Barr; Scirrhia pini Funk; Cytosporina septospora G. Doroguine; Septoriella septospora (G. Dorog.) Sacc.; Actinothyrium marginatum Sacc.; Dothistroma pini var. lineare Thyr; Dothistroma septosporum var. lineare Thyr; Dothistroma pini var. keniense M.H. Ivory; Dothistroma septosporum var. keniense M.H. Ivory; Dothistroma pini sensu auct. NZ; Dothistroma septosporum (G. Dorog.) M. Morelet; Dothistroma septosporum (G. Dorog.) M. Morelet var. septosporum; Septoria septospora (G. Dorog.) Arx.
Classification status: Fungi, Ascomycota, Dothideomycetes, Capnodiales, Mycosphaerellaceae, Mycosphaerella. The asexual form is Dothistroma septosporum (Dorog.) Morelet. See Appendix A for additional information on the pathogen.
4 Principle of the method
This method takes the symptoms of the disease and the morphological, culture and molecular biological characteristics of the fungus as the main basis for quarantine identification.
The host and the geographical distribution are used as a basis for auxiliary identification.
5 Equipment and reagents
5.1 Equipment
Perspective microscope, ultra-clean workbench, electronic balance, incubator, autoclave, constant temperature water bath, pure water meter, vortex oscillator.
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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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Related Standards
SN/T 2122-2008 — Sampling for the quarantine of plants and their products for import and export
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