GB/T 36816-2018Detection and identification of Potato virus Y (English PDF)

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009.

1 Scope

This standard specifies the quarantine and identification methods for potato virus Y.

This standard applies to the quarantine and identification of potato plants, propagation materials and other host plants that may have potato virus Y.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this document. For undated references, the latest edition (including all amendments) applies to this document.

GB 7331 Potato seed potato quarantine regulations

SN/T 1840 plant virus immunoelectron microscopy detection method

SN/T 2122 Quarantine sampling method for inbound and outbound plants and plant products

3 Potato Y virus basic information

Chinese name. Potato Y virus

Scientific name. Potato virus Y

Abbreviation. PVY

Synonym. Potato severe mosaic virus, Potato acropetal necrosis virus, Solanum virus 2, Tobacco vein-banding virus, Potato leaf drop streak virus, Tobacco veinal necrosis virus, Potato virus 20

Classification status. Potyviridae, Potyvirus.

Transmission route. vegetative propagation materials such as potato seed potatoes are transmitted, mechanical vaccination can transmit drugs, and various mites spread the disease in a non-persistent manner. Long-distance transmission is accomplished through the trade of asexual propagation materials such as seed potatoes. According to the data, the potato seed can transmit the virus, but in the raw There is little significance in production; no reports have been reported on the seeds of other Solanaceae crops. See Appendix A for additional information on potato virus Y.

4 Principle of the method

The genomic characteristics, immunogenicity and symptom characteristics of the PVY are the basis for the quarantine and identification of the virus. According to PVY and the genomic features of the main strains were established by reverse transcription-PCR (RT-PCR) and real-time fluorescent reverse transcription-PCR (Real-time RT-PCR) detection method; based on the immunogenicity of PVY and major strains, an enzyme-linked immunosorbent assay (ELISA) method and an immunochromatographic strip tester were established method and immunoelectron microscopy; based on PVY and the characteristic characteristics of major strains on different host plants, the identification of host diagnosis method; a pyrophosphate sequencing method was used to identify strain groups based on the genome sequence of PVY. Through the effective combination of these methods, determine whether the sample carries PVY and major strains.

5 Instruments, equipment and reagents