GB/T 36809-2018Detection and identification of Moniliophthora perniciosa (English PDF)
可可丛枝病菌检疫鉴定方法
This is a limited preview
Buy now to download the full PDF (15 pages)
Need quotation for your management?
Get a formal quotation for GB/T 36809-2018 in 30 minutes.
Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
September 17, 2018
Implementation date
April 1, 2019
Scope
GB/T 36809-2018 is the English-translated version of 可可丛枝病菌检疫鉴定方法.
GB/T 36809-2018 sets out how plant quarantine laboratories detect and identify the cocoa pathogen Moniliophthora perniciosa.
Identification is based on the symptoms on the host, the morphological and culture characteristics of the fungus and its molecular biology, with background on taxonomy, host range, distribution and symptoms given in an appendix.
The standard lists the instruments, from stereo and biological microscopes to PCR, electrophoresis and gel imaging equipment, and the laboratory items needed.
It also specifies the reagents for DNA extraction and PCR and the culture media, including potato dextrose agar and a selective PDA medium, with reference to SN/T 1538.1 for medium preparation. It was issued on 17 September 2018 and has applied since 1 April 2019.
Found what you need?
Document preview — GB/T 36809-2018
National Standard of the People's Republic of China
- ICS
- 65.020.01
- Classification
- B 16
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- Foreword
- 1 Scope
- 2 Normative references
- 3 Principle of the method
- 4 Equipment
- 5 Reagents and media
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
1 Scope
This standard specifies the quarantine and identification methods for Moniliophthora perniciosa.
This standard applies to the quarantine and identification of Moniliophthora perniciosa.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this document. For undated references, the latest edition (including all amendments) applies to this document.
SN/T 1538.1 Guidelines for the preparation of medium - Part 1
3 Principle of the method
The symptoms of the disease on the host and the morphological, culture and molecular biological characteristics of Moniliophthora perniciosa are the main basis of this standard. See Appendix A for background information on the taxonomic status, host range, distribution, and symptoms of the disease.
4 Equipment
4.1 Instrumentation
Stereo microscope, biological microscope, electronic balance (sensitivity 0.001g), autoclave, ultra-clean workbench, biological incubator, PCR instruments, electrophoresis and gel imaging systems.
4.2 Laboratory equipment
Labels, qualitative filter paper, sample bags, storage bags, hand-held magnifiers, tweezers, scalpels, alcohol lamps, inoculation needles, petri dishes (9.0 cm in diameter), beakers, measuring cylinders, flasks, slides, coverslips, adjustable pipettes (adjustable range 2.5µL~1000µL), centrifuge tubes.
5 Reagents and media
5.1 Reagents
Penicillin, streptomycin, absolute ethanol, potato, glucose, agar, 70% ethanol, sterile double distilled water, 2% CTAB extraction buffer, 10% SDS solution, 3mol/L sodium acetate solution, PCR reagent (including Taq polymerase, dNTP, buffer), phenol, chloroform, isopentanol, Tris-HCl, EDTA (ethylenediaminetetraacetic acid), agarose, molecular weight standard, TAE running buffer.
5.2 Medium
Potato dextrose agar medium (PDA): 200 g of peeled potato, 20 g of glucose, 18 g of agar, add water to 1000 mL, autoclave at 121 °C for 20 min.
PDA selective medium: PDA after autoclaving is cooled to 45 °C ~ 50 °C.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
Referenced standards
Similar standards
How to Download GB/T 36809-2018
- 1
Add to cart
Click "Download PDF" on this page and choose "Buy this standard". You can add more standards before checkout.
- 2
Checkout
Enter your email and billing details. Payment is processed securely by Stripe (cards or bank transfer). Prefer bank details? Request a quotation (min. order $300).
- 3
Instant delivery (0–9 sec)
Delivery is automatic: within seconds of payment you'll receive an email with a secure download link. The link stays valid for 72 hours.
- 4
Invoice included
A tax invoice is attached to the confirmation email. Need a custom invoice? Contact us.
Related Standards
SN/T 1538.1-2005 — Guidelines on preparation and production of culture media. Part 1: General guidelines on quality assurance for the preparation of culture media in the laboratory
SN/T 1538.1-2016 — Guidelines on preparation and production of culture media—Part 1: General guidelines on quality assurance for the preparation of culture media in the laboratory
GB/T 10003-2026 — Biaxially oriented polypropylene (BOPP) film
Secure payment via Stripe
Payments accepted
GB/T 36809-2018
$205.00