GB/T 35901-2018Real-time PCR method for detection of porcine circovirus type 2 (English PDF)
猪圆环病毒2型荧光PCR检测方法
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Issued by
State Administration for Market Regulation, China National Standardization Administration
Level / Type
National · Recommended
Issue date
February 6, 2018
Implementation date
September 1, 2018
Scope
GB/T 35901-2018 is the English-translated version of 猪圆环病毒2型荧光PCR检测方法.
GB/T 35901-2018 — This standard specifies the operation of porcine circovirus type 2 fluorescent PCR test. This standard applies to porcine circovirus type 2 nucleic acid testing.
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Document preview — GB/T 35901-2018
National Standard of the People's Republic of China
- ICS
- 11.220
- Classification
- B 41
Issued by: State Administration for Market Regulation, China National Standardization Administration
Contents
- Foreword
- 1 Scope
- 2 Normative references
- 3 Abbreviations
- 4 equipment
- 4.1 Fluorescent PCR detector.
- 4.3 Ordinary refrigerator. 2 degrees C ~ 8 degrees C.
- 4.4 Ordinary freezer. -20 degrees C below.
- 4.5 ultra-low temperature refrigerator. temperature control can be -70 degrees C below.
- 4.6 Micropipettor. 0.2µL ~ 2µL, 1µL ~ 10µL, 10µL ~ 100µL, 20µL ~.200µL, 100µL ~ 1000µL and
- 4.7 autoclave.
- 5 supplies
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the National Animal Husbandry Standardization Technical Committee (SAC/TC181) centralized.
This standard was drafted. Shanghai Entry-Exit Inspection and Quarantine of the People's Republic of China, Shanghai Institute of Veterinary Medicine, Chinese Academy of Agricultural Sciences, China
Shenzhen Entry-Exit Inspection and Quarantine of People's Republic of China, China Inspection and Quarantine Science Institute, Hunan Shengxiang Biological Technology Co., Ltd.
1 Scope
This standard specifies the operation of porcine circovirus type 2 fluorescent PCR test.
This standard applies to porcine circovirus type 2 nucleic acid testing.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this document. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 analytical laboratory water specifications and test methods
GB/T 25172 pig sperm production and preservation of technical specifications
3 Abbreviations
The following abbreviations apply to this document.
Fluorescent PCR. Fluorescent polymerase chain reaction (real-time polymerase chain reaction)
Ct value. the number of cycles in each reaction tube fluorescent signal reaches the set threshold value (cyclethreshold)
DNA. deoxyribonucleic acid
Taq enzyme. Taq DNA polymerase (Taq DNA polymerase)
TE buffer. Tris-EDTA buffer (Tris-EDTA buffer)
PCV. porcine circovirus
4 equipment
4.1 Fluorescent PCR detector.
4.2 high-speed desktop refrigerated centrifuge. temperature can be controlled to 4 degrees C, centrifuge speed up to 12000r/min above.
4.3 Ordinary refrigerator. 2 degrees C ~ 8 degrees C.
4.4 Ordinary freezer. -20 degrees C below.
4.5 ultra-low temperature refrigerator. temperature control can be -70 degrees C below.
4.6 Micropipettor. 0.2µL ~ 2µL, 1µL ~ 10µL, 10µL ~ 100µL, 20µL ~.200µL, 100µL ~ 1000µL and
Equipped with tips that match the pipette.
4.7 autoclave.
5 supplies
5.1 1.5mL centrifuge tube.
5.2 0.2mLPCR thin-walled tube or octagonal tube.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 17 pages — is available in the English PDF.
Referenced standards
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