GB/T 25786-20102-Amino-4-acetamino anisole (English PDF)

5.3 Determination of content of 2-Amino-4-acetamido anisole (amino value,

calculated in dry product)

5.3.1 Reagent and solvent

a) Hydrochloric acid solution. Volume ratio of hydrochloric acid to water is 1.1;

b) Sodium nitrite standard titration solution. c(NaNO2)=0.1mol/L, during calibration,

use starch-potassium iodide test paper to determine the end point;

c) Potassium bromide solution. 100g/L;

d) Starch - potassium iodide test paper.

5.3.2 Analytical steps

Weigh about 0.5g (accurate to 0.1mg) of sample; place in a 400mL beaker; add 200mL of

hot water to dissolve it; add 30mL of 1+1 hydrochloric acid solution and 20mL of potassium

bromide solution; cool to 5°C ~ 10°C; use sodium nitrite standard solution to perform

titration till the potassium iodide starch test paper appears in light blue, and lasts for 5min

without disappearing, that is the end point; perform blank test at the same time.

5.3.3 Result calculation

Content of 2-Amino-4-acetamido anisole (amino value, calculated in dry product), counted

in mass fraction w1, its value is expressed in %, is calculated according to Formula (1).

Where.

V - The consumption value of standard sodium nitrite volumetric solution volume for

specimen, in unit of milliliter (mL);

V0 - The consumption value of standard sodium nitrite titration solution for blank test,

in unit of milliliter (mL);

c - The actual value of the standard sodium nitrite titration solution concentration, in

unit of mole per liter (mol/L);

M - Molar mass of 2-Amino-4-acetamido anisole, in unit of gram per mole (g/mol

[M(C9H12N2O2)=180.20];

m - The value of test sample's mass (calculated in dry product), in unit of gram (g).

Calculation result is rounded to two decimal places.

5.3.4 Permissible error

e) Sample injection. 5µL.

Choose the appropriate operation conditions of chromatogram according to different

instruments.

5.4.5 Analytical steps

Weigh about 25mg of sample (accurate to 1mg); place it in a 25mL volumetric flask; add

methanol to dissolve and fix the volume; place in an ultrasonic generator to dissolve fully;

take it out; shake well for standby application.

After the instrument operates stably, use a micro syringe to absorb the sample solution to

inject into an injection valve; perform the result processing after the last component flows

out (see Figure 1).

5.4.6 Result calculation

The purity of 2-Amino-4-acetamido anisole, counted in w2, its value is expressed in %, is

calculated according to Formula (2).

Where,

A - The peak area value of 2-Amino-4-acetamido anisole;

sumAi - A sum of peak area values of various components in the sample.

Calculation result is rounded to two decimal places.

5.4.7 Permissible error

The difference between two parallel determination results shall not be greater than 0.2%;

take the arithmetic mean of them as the determination result.

5.4.8 Chromatogram

Chromatogram is shown in Figure 1.

7.1 Marks and labels

Each package of 2-Amino-4-acetamido anisole shall be coated with a firm and clearly

marked, and be noted with product name, specification, registered trademark, product

license number, logo (if applicable), net content, manufacturer name and address,

standard number, batch number, and production date. Batch number and production date

can also be printed on the label. The label and product qualification certificate shall be

placed outside the plastic bag in the packing barrel.

7.2 Packaging

2-Amino-4-acetamido anisole is packaged with a woven bag that is lined with a plastic bag;

net weight of each pad is 50kg. Other packaging can be determined through negotiation

with the user.

7.3 Transport

During transport, it shall prevent solarization, dampness and rain, and avoid strong

squeezing and collision.

7.4 Storage

2-Amino-4-acetamido anisole shall be stored in a cool, dry and ventilated storeroom,

prevented from dampness and heating, kept away from the fire, and stored and transported

according to provisions of chemicals.

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