GB/T 22983-2008Determination of six polyether antibiotics residues in milk and milk powder - LC-MS-MS method (English PDF)

Foreword

The Standard Appendix A, Appendix B is an informative annex. This standard is proposed and managed by the State Administration of Quality Supervision Inspection and Quarantine of People's Republic of China. This standard was drafted. People's Republic of China Qinhuangdao Exit Inspection and Quarantine, Qinghai People's Republic of China Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. thin Hyperion, Caoyan Zhong, Luo Lili, Jia light group, star jade show, Li communication Pang Guofang. Milk and milk powder of Six Polyether Antibiotics Determination of residues Liquid chromatography - tandem mass spectrometry

1 Scope

GB/T 22983-2008 is the Chinese national standard on determination of six polyether antibiotics residues in milk and milk powder - lc-ms-ms method. The /T suffix marks it as a recommended standard: it is not compulsory by itself, but it becomes binding as soon as a contract, a tender or a customer specification calls it up - which in practice is how most foreign buyers meet it. It was issued on 31 December 2008 by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China; Standardization Administration of China, and has been in force since 1 May 2009. Classification: CCS X04. This page is published from the official record of the standard held by the Chinese standards administration: the identification, the dates, the classification and the issuing body are taken from there. The clause text, the tables and the numeric limits are in the document itself, which is delivered complete in English translation.

This standard specifies the milk and milk powder of Six Polyether Antibiotics Residues of liquid chromatography - tandem mass spectrometry. This standard applies to liquid milk (including raw milk, whole milk, skim milk) and milk (including plain milk, skim milk powder and infant formula Milk) in La Shaluo streptozotocin (lasalocid), monensin (monensin), Nigeria streptozotocin (nigericin), salinomycin (salinomycin), Narasin (narasin), maduramicin ammonium (madubamycinammonium) Determination of the residual. The standard method detection limit. milk Lasha Luo, monensin, Nigeria streptozotocin, salinomycin, narasin, maduramicin Ammonium detection limits were 0.2µg/L; milk powder Lasha Luo, monensin, Nigeria streptozotocin, salinomycin, narasin, maduramicin Ammonium detection limits were 1.6µg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions ( GB/T 6379.1-2004, ISO 5725-1.1994, IDT)

GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat Of the basic methods of reproduction ( GB/T 6379.2-2004, ISO 5725-2.1994, IDT)

GB/T 6682 analytical laboratory use specifications and test methods ( GB/T 6682-2008,

ISO 3696. 1987, MOD) Principle

3 Polyether antibiotics sample with acetonitrile extraction, solid-phase extraction (SPE), high performance liquid chromatography - tandem mass spectrometer, external standard.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade.

4.1 Water. GB/T 6682, level.

4.2 acetonitrile. chromatography.

4.3 Methanol. chromatographically pure.

4.4 hexane. chromatographically pure.

4.5 n-hexane saturated acetonitrile. (4.4) in a small amount of acetonitrile (4.2) was added n-hexane and mix thoroughly. After resting stratification, take the upper n Hexane.

4.6 formic acid. pure class distinctions.

4.7 ammonium acetate. pure class distinctions.

4.8 anhydrous sodium sulfate. before scorching 4h at 650 °C, placed after the dryer to cool reserve.

4.9 aqueous mobile phase. 1mL 0.385g formic acid and ammonium acetate dissolved in 1000mL water, and mix. Day of preparation.

4.10 methanol solution (1 + 1). 100mL of methanol and 100mL of water were mixed uniformly.