GB/T 22975-2008Determination of amoxicillin, ampicillin, piperacillin, penicillin G, penicillin V, oxacillin, cloxacillin, nafcillin, dicloxacillin residues in milk and milk powder - LC-MS-MS method (English PDF)

Foreword

The Standard Appendix A, Appendix B is an informative annex. This standard is proposed and managed by the State Bureau of Quality Supervision, People's Republic of China. This standard is drafted by: People's Republic of China Qinhuangdao Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Hebei Entry-Exit Inspection Quarantine Bureau. Drafters of this standard. segment Wenzhong, Jia Haitao, Yi Lianfeng, Chenrui Chun, Guo Chunhai, Mayu Song, Liu Sheng, Cui Yijie, Pang Guofang. Milk and milk powder amoxicillin, ampicillin, piperazine pull Amoxicillin, penicillin G, penicillin Vp, oxacillin, Cloxacillin, nafcillin and dicloxacillin Determination of residues Liquid chromatography - tandem mass spectrometry

1 Scope

GB/T 22975-2008 is the Chinese national standard on determination of amoxicillin, ampicillin, piperacillin, penicillin g, penicillin v, oxacillin, cloxacillin, nafcillin, dicloxacillin residues in milk and milk powder - lc-ms-ms method. The /T suffix marks it as a recommended standard: it is not compulsory by itself, but it becomes binding as soon as a contract, a tender or a customer specification calls it up - which in practice is how most foreign buyers meet it. It was issued on 31 December 2008 by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China; Standardization Administration of China, and has been in force since 1 May 2009. Classification: ICS (ICS) , CCS X04. This page is published from the official record of the standard held by the Chinese standards administration: the identification, the dates, the classification and the issuing body are taken from there. The clause text, the tables and the numeric limits are in the document itself, which is delivered complete in English translation.

This standard specifies the milk and milk powder amoxicillin, ampicillin, piperacillin, penicillin G, penicillin V, oxacillin, chlorazol West Lin, nafcillin and dicloxacillin residues by high performance liquid chromatography - tandem mass spectrometry. This standard applies to milk and milk powder amoxicillin, ampicillin, piperacillin, penicillin G, penicillin V, oxacillin, chlorazol West Determination of forest, nafcillin and dicloxacillin residues and confirmation. The standard method detection limit. milk ampicillin, nafcillin was 1µg/kg, amoxicillin, piperacillin, penicillin G, penicillin Su V, cloxacillin of 2µg/kg, oxacillin, dicloxacillin of 4µg/kg; milk ampicillin, nafcillin to 8µg/kg, amoxicillin Amoxicillin, piperacillin, penicillin G, penicillin V, cloxacillin was 16µg/kg, oxacillin, dicloxacillin to 32µg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions ( GB/T 6379.1- 2004, ISO 5725-1.1994, IDT)

GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat Of the basic methods of reproduction ( GB/T 6379.2-2004, ISO 5725-2.1994, IDT)

GB/T 6682 analytical laboratory use specifications and test methods ( GB/T 6682-2008,

ISO 3696. 1987, MOD) Principle

3 With acetonitrile - water extracted sample analyte solid-phase extraction (SPE), high performance liquid chromatography - tandem mass spectrometry, external standard.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations.

4.1 acetonitrile. liquid chromatography.

4.2 acetic acid. liquid chromatography.

4.3 dibasic sodium phosphate.

4.4 sodium hydroxide. 4.5 5mol/L sodium hydroxide solution. 100g of sodium hydroxide (4.4) dissolved in 450mL of water, add water volume to 500mL. 4.6 0.1mol/L phosphate buffer solution. 6g disodium hydrogen phosphate (4.3) were dissolved in 450mL of water (4.5) adjusted with sodium hydroxide solution Section pH = 8, add water to 500mL, before using the preparation.

4.7 acetonitrile - water (3 + 1). 300mL acetonitrile (4.1) was mixed with 100mL of water.