GB/T 22965-2008Determination of 12beta-agonists residues in milk and milk powder - LC-MS-MS method (English PDF)

Foreword

The Standard Appendix A, Appendix B is an informative annex. This standard is proposed and managed by the State Administration of Quality Supervision, Inspection and Quarantine. This standard was drafted. People's Republic of China Qinhuangdao Exit Inspection and Quarantine, People's Republic of China Fujian Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Yang Fang, Liu Cai, Lin Yonghui, I Contier, Huang Jie, Liyao Ping, Chen Jian, Pang Guofang. Milk and milk powder 12 kinds of beta- agonists Determination of residues Liquid chromatography - tandem mass spectrometry

1 Scope

GB/T 22965-2008 is the Chinese national standard on determination of 12beta-agonists residues in milk and milk powder - lc-ms-ms method. The /T suffix marks it as a recommended standard: it is not compulsory by itself, but it becomes binding as soon as a contract, a tender or a customer specification calls it up - which in practice is how most foreign buyers meet it. It was issued on 31 December 2008 by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China; Standardization Administration of China, and has been in force since 1 May 2009. Classification: ICS (ICS) , CCS X04. This page is published from the official record of the standard held by the Chinese standards administration: the identification, the dates, the classification and the issuing body are taken from there. The clause text, the tables and the numeric limits are in the document itself, which is delivered complete in English translation.

This standard specifies the milk and milk powder 12 kinds of beta- agonists residues in liquid chromatography - tandem mass spectrometry. This standard applies to 12 kinds of multi-residue determination of beta- agonists milk and milk powder. 12 kinds of stimulant medications include bromine Botero beta- (Brombuterol), Zeeman Castro (cimaterol), clenbuterol (clenbuterol), clenbuterol Pointe (clenpenterol), hydroxymethyl ammonia clenbuterol Castro (hydroxymethylclenbuterol), phenoxy propofol amine (isoxsuprine), Maputo Castro (mabuterol), ractopamine (Ractopamin), ritodrine (ritodrine), albuterol (salbutamol), terbutaline (terbutaline) and tulobuterol (Tulobuterol). The method detection limit of this standard. milk ractopamine, salbutamol, Zeeman Castro, Pant and clenbuterol clenbuterol detection limit 0.05µg/kg, bromine Botero, tulobuterol, Maputo Castro, terbutaline, ritodrine, phenoxy propofol amine and ammonia hydroxymethyl clenbuterol detection Limit was 0.25µg/kg; milk powder ractopamine, salbutamol, Zeeman Castro, Pant and clenbuterol clenbuterol detection limit 0.4µg/kg, bromine Botero, tulobuterol, Maputo Castro, terbutaline, ritodrine, phenoxy propofol amine and ammonia hydroxymethyl clenbuterol detection Limit was 2.0µg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions ( GB/T 6379.1-2004, ISO 5725-1.1994, IDT)

GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat Of the basic methods of reproduction ( GB/T 6379.2-2004, ISO 5725-2.1994, IDT)

GB/T 6682 analytical laboratory use specifications and test methods ( GB/T 6682-2008,

ISO 3696. 1987, MOD) Principle

3 Dilute acid hydrolysis samples after protein precipitation with perchloric acid, the residue of beta- agonists with ethyl acetate and isopropyl alcohol mixed solvent, mixed Cation exchange adsorption inverted solid phase extraction (SPE), liquid chromatography - tandem mass spectrometry, internal standard.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations.

4.1 Methanol. chromatographically pure.

4.2 isopropanol.

4.3 ethyl acetate.

4.4 acetonitrile. chromatography.

4.5 formic acid.

4.6 perchloric acid.