GB/T 22595-2008Test method for efficacy of antimicrobials - Aerobic bacteria (English PDF)

Foreword

Appendix A and Appendix B of this Standard are normative. This Standard was proposed by China Petroleum and Chemical Industry Federation. This Standard shall be administrated by Water Treatment Sub-committee of National Standardization Technical Committee (SAC/TC 63/SC 5). Responsible drafting organizations of this Standard. Shanghai Future Enterprises Limited, and CNOOC Tianjin Chemical Research and Design Institute. Main drafters of this Standard. Liu Xin, Zhang Quan, Shao Hongqian, and Zhu Chuanjun. Test method for efficacy of antimicrobials - Aerobic bacteria

1 Scope

GB/T 22595-2008 is the Chinese national standard on test method for efficacy of antimicrobials - aerobic bacteria, in the field of testing. The /T suffix marks it as a recommended standard: it is not compulsory by itself, but it becomes binding as soon as a contract, a tender or a customer specification calls it up - which in practice is how most foreign buyers meet it. It was issued on 23 December 2008 by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China; Standardization Administration of China, and has been in force since 1 September 2009. Classification: ICS 19.020, CCS G76. This page is published from the official record of the standard held by the Chinese standards administration: the identification, the dates, the classification and the issuing body are taken from there. The clause text, the tables and the numeric limits are in the document itself, which is delivered complete in English translation.

This Standard specifies the test method for the sterilization efficacy of aerobic bacteria by using antimicrobial in cooling water system. This Standard applies to the test for the sterilization efficacy of oxidizing and non-oxidizing antimicrobial to the aerobic bacteria.

2 Normative references

The provisions in following documents become the provisions of this Standard through reference in this Standard. For dated references, the subsequent amendments (excluding corrections) or revisions do not apply to this Standard, however, parties who reach an agreement based on this Standard are encouraged to study if the latest versions of these documents are applicable. For undated references, the latest edition of the referenced document applies.

GB/T 603 Chemical reagent - Preparations of reagent solutions for use in test methods ( GB/T 603-2002, ISO 6353-11982, NEQ)

GB/T 6682 Water for analytical laboratory use - Specification and test methods ( GB/T 6682-2008, ISO 3696. 1987, MOD)

GB/T 14643.1 Industrial circulating cooling water - Examination of bacteria formed deposits - Standard of plate count

3 Summary of methods

In the sample that contains a certain amount of aerobic bacteria. ADD antimicrobials quantitatively. SIMULATE antimicrobial's use conditions (water quality, pH value, etc.). DETERMINE the amount of aerobic bacteria survived in the prescribed time period and COMPARE it with the amount of aerobic bacteria at the beginning of test. CALCULATE the killing rate of this cooling water, the selection range of concentration of the antimicrobial to be added shall be in accordance with the characteristics and use conditions of the antimicrobial. CHOOSE several concentration grades in a certain range to conduct test.

7.1.3 To make dosing operation more convenient, the original fluid of antimicrobial to be tested shall be diluted with water before the test. The degree of dilution shall be based on - after the diluted solution of antimicrobial is added to the sample, it just reaches the concentration needed in test (the ratio of the volume of diluted antimicrobial and the volume of sample to be added shall not exceed 1.100). The diluted solution of antimicrobial shall be used immediately after it is diluted.