GB/T 19495.9-2017Detection of genetically modified organisms and derived products—Liquid bead array detection for plant products (English PDF)

Foreword

GB/T 19495 "Genetically Modified Products Testing" has released or plans to release the following sections.

--- GB/T 19495.1 Genetically Modified Products Testing General Requirements and Definitions;

--- GB/T 19495.2 Genetically Modified Products Testing Laboratory technical requirements;

--- GB/T 19495.3 GMO product detection nucleic acid extraction and purification methods;

--- GB/T 19495.4 GMO products detection of nucleic acid qualitative PCR detection method;

--- GB/T 19495.5 GMO products for detection of nucleic acid quantitative PCR detection method;

--- GB/T 19495.6 Genetically Modified Products Detection of gene chip detection methods;

--- GB/T 19495.7 Genetically Modified Products Sampling and sample preparation methods;

--- GB/T 19495.8 Genetically Modified Products for Detection of Protein Detection Methods;

--- GB/T 19495.9 Genetically Modified Products Testing of plant products liquid chip detection methods;

--- GB/T 19495.10 Genetically Modified Products General Test Method for Plant Products;

--- GB/T 19495.11 Genetically Modified Products Testing of plant products digital PCR detection method;

--- GB/T 19495.12 GMO detection of alfalfa real-time fluorescent PCR detection method.

This section is GB/T 19495 9 parts.

This section drafted in accordance with GB/T 1.1-2009 given rules.

1 Scope

GB 19495 provisions of this part of the qualitative detection of liquid components of the genetically modified chips.

This section applies to corn, soybeans, rapeseed, rice, cotton and other transgenic plants and their products CaMV35S and NOS gene qualitative Detection.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this document Pieces. For undated references, the latest edition (including all amendments) applies to this document.

GB/T 6682 analytical laboratory water specifications and test methods

GB/T 19495.1 Genetically Modified Products General Requirements and Definitions

GB/T 19495.3 Genetically modified products for nucleic acid extraction and purification methods

GB/T 19495.7 Genetically Modified Products - Sampling and Sample Preparation Methods

3 Abbreviations

The following abbreviations apply to this document.

PCR. Polymerase Chain Reaction (Polymerase Chain Reaction)

MFI. Median Fluorescence Intensity

SA-PE. Streptavidin-Phycoerythrin

4 Principle

Liquid-chip technology to fluorescent-encoded microspheres based microspheres with a large number of active surface groups, with nucleic acid probes, antigens, antibodies, etc. Molecular coupling. Microspheres in the preparation process incorporates two kinds of red and orange dyes in proportion to mix the fluorescent dye, two kinds of dye points Different proportions are given to the microspheres of different colors, which microspheres are divided into many kinds, each microsphere-specific coupling for the purpose of DNA Sequence of oligonucleotide probes, it is possible to label 100 different probe molecules. Then add the target molecules and fluorescence with the report Molecules, probe molecules on different microspheres and different molecules to achieve specific binding, the formation of a flexible liquid-chip system. This The method for signal recognition and detection is flow cytometry, which rapidly arranges microspheres in single columns through the detection channel using red and green The two laser beams illuminate the individual microspheres. The red laser classifies the microspheres by identifying the spectral fingerprint of the microspheres themselves The green laser detects the molecular weight of the bound fluorescent reporter on the microspheres to quantify the reaction. The resulting fluorescence signal is photoimaged After the tube by the computer processing, the final analysis of the data, the results. When testing, liquid-phase chip detector records only two kinds of fluorescence at the same time Current fluorescent signals do not record unbound fluorescent reporter signals.

This standard for universal screening element CaMV35S promoter and NOS terminator designed to select specific primers and probes for liquid phase Chip detection, and based on test results to determine the CaMV35S promoter and NOS terminator exogenous screening element components.