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GB/T 13277.7-2021Compressed air - Part 7: Test method for viable microbiological contaminant content (English PDF)

压缩空气 第7部分:活性微生物含量测量方法压缩空气 第7部分:活性微生物含量测量方法

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Issued by

State Administration for Market Regulation, China National Standardization Administration

Level / Type

National · Recommended

Issue date

December 31, 2021

Implementation date

July 1, 2022

Scope

GB/T 13277.7-2021 is the English-translated version of 压缩空气 第7部分:活性微生物含量测量方法压缩空气 第7部分:活性微生物含量测量方法.

GB/T 13277.7-2021 is the Chinese standard "Compressed air - Part 7: Test method for viable microbiological contaminant content".

Its scope clause reads: This document specifies procedures for determining the presence, sampling, culturing and quantifying the presence of viable microorganisms (e:g: yeast, bacteria, endotoxins) experiment method: This document applies to the evaluation of purification levels according to GB/T 13277:1, and also applies to the determination of solid particles in combination with GB/T 13277:4: active colony-forming units:. Its clauses include terms and definitions.

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Document preview — GB/T 13277.7-2021

National Standard of the People's Republic of China

Classification
J 72

Issued by: State Administration for Market Regulation, China National Standardization Administration

Contents

  • Preface
  • Introduction
  • 1 Scope
  • 2 Normative references
  • 3 Terms and Definitions
  • 4 Method 2 to determine the presence of viable microorganisms by partial flow sampling
  • 5 Working condition
  • 6 Determination of viable colony microorganisms
  • 7 Test report
  • Appendix A (normative) Quantitative sampling method
  • Appendix B (Informative) Endotoxin Sampling
  • Appendix C (Informative) Preparation of Petri Dish with Medium
  • Appendix D (Informative) Measurement of Quantity of Active Microorganisms in Compressed Air --- Sampling Test Report

1 Scope

This document specifies procedures for determining the presence, sampling, culturing and quantifying the presence of viable microorganisms (e:g: yeast, bacteria, endotoxins) experiment method:

This document applies to the evaluation of purification levels according to GB/T 13277:1, and also applies to the determination of solid particles in combination with GB/T 13277:4:

active colony-forming units:

2 Normative references

The contents of the following documents constitute essential provisions of this document through normative references in the text: Among them, dated citations documents, only the version corresponding to that date applies to this document; for undated references, the latest edition (including all amendments) applies to this document:

GB/T 13277:1 Compressed Air Part 1: Pollutant Purification Level (GB/T 13277:1-2008, ISO 8573-1:2001,

MOD)

GB/T 13277:4 Compressed Air Part 4: Method for the Measurement of Solid Particles (GB/T 13277:4-2015, ISO 8573-4:

2001, MOD)

3 Terms and Definitions

The following terms and definitions apply to this document:

3:1 microbiologicalorganisms

Particles capable of forming active flora:

NOTE: These can be identified as bacteria, yeast or fungi:

3:2 numberofviablemicro-organisms

The number of microorganisms with the potential for metabolic activity:

3:3 culturablenumber

The number of aggregates, single cells or microorganisms that can form colonies on a solid nutrient medium:

3:4 colony-forming unit

CFU A unit that represents the culturable quantity: […]

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 22 pages — is available in the English PDF.

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