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GB 5009.206-2016Determination of tetrodotoxin in aquatic products -- HPLC-fluorescence detection method (English PDF)

食品安全国家标准水产品中河豚毒素的测定

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Issued by

National Health and Family Planning Commission People's Republic of China

Level / Type

National · Mandatory

Issue date

December 23, 2016

Implementation date

June 23, 2017

Scope

GB 5009.206-2016 is the English-translated version of 食品安全国家标准水产品中河豚毒素的测定.

GB 5009.206-2016 — This standard specifies the method for the determination of tetrodotoxin in aquatic products. The standard method for the determination of a first puffer fish muscle, liver, skin and gonad tissues tetrodotoxin.

The second method is suitable for puffer fish Muscle, liver measured, tetrodotoxin skin and gonads. The third method is suitable for puffer fish, snails, shrimp, oysters, squid, and Clam Determination of tetrodotoxin.

The fourth method is suitable for determination of puffer fish muscle, liver, skin and gonad tissues tetrodotoxin.

The first mouse bioassay method Principle 2 the principle of tetrodotoxin soluble in an acidic solution, the sample was prepared acetic acid solution twice (0.5%) boiling extraction, centrifugation 20000g the supernatant was collected and the two supernatants were combined volume to 25mL, a mouse bioassay.

The mice died after injection of the sample extract Time, isolated rat Units and weight correction unit mice rats, calculated to determine the content of tetrodotoxin.

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Document preview — GB 5009.206-2016

National Standard of the People's Republic of China

Classification: X 04
Replacing: GB/T 23217-2008; GB/T 5009.206-2007; SN/T 1569.2-2013

Issued by: National Health and Family Planning Commission People's Republic of China

Document data1

Document data

Chinese classification (CCS): X04.

Estimated length of the Chinese original: 20,267 words.

Supersedes the earlier edition GB/T 23217-2008; GB/T 5009.206-2007; SN/T 1569.2-2013.

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 13 pages — is available in the English PDF.

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