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GB 5009.148-2014National Food Safety Standard -- Determination of Free Gossypol in Vegetable Food (English PDF)

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Issued by

National Health and Family Planning Commission of PRC

Level / Type

National · Mandatory

Issue date

February 4, 2015

Implementation date

May 1, 2015

Scope

GB 5009.148-2014 (National Food Safety Standard -- Determination of Free Gossypol in Vegetable Food) is available as an English-translated PDF.

GB 5009.148-2014 is the Chinese standard "National Food Safety Standard -- Determination of Free Gossypol in Vegetable Food". Its scope clause reads: This standard specifies the method for determination of free gossypol in vegetable oil or other liquid foods using cottonseed cake as raw material.

This standard applies to the determination of free gossypol in vegetable oil or other liquid foods using cottonseed cake as raw material. Its clauses include principle; reagents and materials; expression of analysis results.

It was issued by the National Health and Family Planning Commission of PRC on 2015-02-04 and took effect on 2015-05-01.

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Document preview — GB 5009.148-2014

National Standard of the People's Republic of China

Issued by: National Health and Family Planning Commission of PRC

Foreword...3
1 Scope...4
2 Principle...4
3 Reagents and materials...4
4 Instrument and equipment...5
5 Analytical procedures...5
6 Expression of analysis results...6
7 Precision...7
8 Others...7
Appendix A Standard solution of gossypol and sample spiked chromatogram8

1 Scope

This standard specifies the method for determination of free gossypol in vegetable oil or other liquid foods using cottonseed cake as raw material.

This standard applies to the determination of free gossypol in vegetable oil or other liquid foods using cottonseed cake as raw material.

2 Principle

The free gossypol in vegetable oil was extracted by absolute ethanol and detected by high performance liquid chromatography. The retention time of the chromatographic peak is qualitatively determined, and it is quantified by external standard method.

The free gossypol in the water-soluble liquid sample using cottonseed cake as the raw material is extracted by the use of anhydrous ether, concentrated to dryness, then dissolved in ethanol, detected by high performance liquid chromatography. The retention time of the chromatographic peak is qualitatively determined, and it is quantified by external standard method.

3 Reagents and materials

Note: Unless otherwise specified, the reagents used in this method are all analytically pure; the water is the level 1 water as specified in GB/T 6682.

3.1 Reagents

3.1.1 Phosphoric acid (H3PO4).

3.1.2 Anhydrous ethanol (CH3CH2OH).

3.1.3 Acetone (C3H6O).

3.1.4 Nitrogen (N2).

3.1.5 Methanol (CH3OH).

upper layer of ether. Use nitrogen to blow it dry. Use 1.0 mL of absolute ethanol to make its volume reach to the mark. Make it pass through a 0.45 µm filter membrane, which is the sample solution.

5.2 Chromatographic determination

5.2.1 Reference chromatographic conditions

Chromatographic column: C18 column, 250 mm × 4.6 mm, 5 µm, or a column with equivalent performance.

Mobile phase: Methanol: phosphoric acid solution = 85: 15.

Flow rate: 1.0 mL/min.

Column temperature: 40 °C ± 1 °C.

Measurement wavelength: 235 nm.

Sample injection volume: 10 µL.

5.2.2 Drawing standard curve

Inject the standard working solution of gossypol into the high performance liquid chromatograph and record the peak height or peak area. Draw a standard curve with the peak height or peak area as the ordinate and the concentration of the standard working solution of gossypol as the abscissa (see Figure A.1 in

Appendix A for the liquid chromatogram of the standard solution).

5.2.3 Specimen measurement

Inject the specimen solution into the high performance chromatograph. Record the peak height or peak area. Calculate the concentration of gossypol in the test solution based on the standard curve (see Figure A.2 in Appendix A for the sample spiked liquid chromatogram).

6 Expression of analysis results

The free gossypol content in specimen 5.1.1 is calculated according to formula (1):

Where:

X - The content of gossypol in the specimen, in milligrams per kilogram

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 8 pages — is available in the English PDF.

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