GB 31625-2014National Food Safety Standard -- Food Additives -- Methyl dihydrojasmonate (English PDF)

2.1 Chemical Name

(2-pentyl-3-oxo-cyclopentyl) - acetic acid methyl ester Formula 2.2 C13H22O3

2.3 Formula

2.4 Molecular Weight

226.32 (according to 2007 international relative atomic mass)

3.1 Sensory requirements

Shall comply with the requirements in Table 1.

Table 1 Sensory requirements

Project requires test methods Color colorless to pale yellow State oily liquid the sample was placed in a cuvette, were visually observed the aroma of flowers, jasmine-like aroma GB/T 14454.2

3.2 Physical and Chemical Indicators

Shall be in accordance with Table 2.

Table 2. Physical and chemical indicators

Item Index Test Method Dihydro jasmonate content (w) /% Trans >= 85 Cis >= 9 ~ 11 Appendix A, Appendix B Acid number (as KOH)/(mg/g) <= 2.0 GB/T 14455.5 Refractive index (20 °C) 1.454 ~ 1.464 GB/T 14454.4 Relative density (20 °C/20 °C) 0.997 ~ 1.008 GB/T 11540 Appendix A Determination of Content dihydro jasmonate A.1 instruments and equipment A.1.1 Chromatography. in GB/T 11538-2006 specified in Chapter 5.

A.1.2 Column. capillary column.

A.1.3 Detector. hydrogen flame ionization detector.

A.2 Determination

Area normalization method. Press the GB/T 11538-2006 Determination 10.4.

A.3 repeatability and results are expressed 11538-2006 carried out in accordance with the provisions of 11.4 GB/T , should meet the requirements.

Food additives dihydro jasmonate typical gas chromatogram (area normalization method) Appendix B.

Appendix B

Food additives dihydro jasmonate typical gas chromatogram (Area normalization method) B.1 dihydro jasmonate food additives typical gas chromatogram Food additives dihydro jasmonate typical gas chromatogram shown in Figure B.1.

Description.

A --- trans-dihydro jasmonate.

B --- cis-dihydro jasmonate.

Figure B.1 dihydro jasmonate food additives typical gas chromatogram

B.2 operating conditions B.2.1 Column. capillary column, 30m × 0.32mm. Or other equivalent column.

B.2.2 stationary phase. 5% phenyl methyl polysiloxane.

B.2.3 film thickness. 0.25µm.

B.2.4 chromatographic oven temperature. program from 100 °C ~ 220 °C, a rate of 5 °C/min, and finally held at 6min 220 °C.

B.2.5 Inlet temperature. 250 °C.

B.2.6 Detector temperature. 280 °C.

B.2.7 Detector. hydrogen flame ionization detector.

B.2.8 Carrier gas. nitrogen purity of 99.99% or more.

B.2.9 carrier gas flow rate. 1.0mL/min.

B.2.10 Injection volume. 0.2µL.

B.2.11 split ratio. 1.80.