GB 31604.18-2016National food safety standard - Food contact materials and articles - Determination of migration of acrylamide (English PDF)

Foreword

This standard replaces GB/T 23296.9-2009 "Food contact materials - Polymer

- Determination of acrylamide in food simulants - High performance liquid

chromatography".

As compared with GB/T 23296.9-2009, the main changes of this standard are

as follows.

- CHANGE the standard name into "National food safety standard - Food

contact materials and articles - Determination of migration of acrylamide";

- ADD the scope of application;

- MODIFY the food simulant preparation;

- MODIFY the processing of oil-based food simulant test solutions.

National food safety standard - Food contact materials

and articles - Determination of migration of acrylamide

1 Scope

This standard specifies the determination of the migration of acrylamide in food

contact materials and articles.

This standard applies to the use of liquid chromatography for the detection of

acrylamide migration in food contact materials and articles.

2 Principle

The food simulants of food contact materials and articles are tested by liquid

chromatography, wherein the water-based, acidic, and alcoholic food simulants

are directly injected, the oil-based food simulants are injected after water

extraction and separated through high-performance liquid chromatography.

(The column is an ion exclusion column), detected by a UV detector, and it is

quantified using the external standard peak area method.

3 Reagents and materials

Unless otherwise stated, the reagents used in this method are of analytical

grade, and the water is grade I water as specified in GB/T 6682. Containers

and transfer apparatus used in the test shall avoid the use of plastic materials.

5 colorimetric tubes, respectively PIPETTE 0.00 mL, 0.03 mL, 0.05 mL, 0.08

mL, 0.1 mL and 0.5 mL of acrylamide standard intermediate solution (10 mg/L)

in the colorimetric tube, MIX it uniformly, ADD 10.0 mL of water, SHAKE it

vigorously for 1 min, LET it be standing for stratification. The acrylamide

concentration of the obtained standard working solution is respectively 0.00

mg/kg, 0.03 mg/kg, 0.05 mg/kg, 0.08 mg/kg, 0.10 mg/kg, and 0.50 mg/kg. TAKE

the lower layer aqueous solution, MAKE it pass through a 0.2 µm filter

membrane for measurement.