GB 31602-2015National food safety standard -- Dried sea cucumber (English PDF)

2.1 Dried sea cucumber

Products made from sea cucumbers such as stichopus japonicus as raw materials, which have undergone the processes of gutting, cooking, salting (or not), desalting (or not), and drying; or using salted sea cucumbers as raw materials, which have undergone the processes of desalting (or without desalination), drying and the like.

NOTE. During the harvest season of stichopus japonicus, the usual practice is to boil and salt the fresh sea cucumbers to make semi-finished products (that is, salted sea cucumbers); and store them in cold storage as raw materials for the production of dried sea cucumbers.

2.2 Dry weight ratio after rehydration

The percentage of dry matter mass obtained by drying the dried sea cucumber after rehydration.

3.1 Sensory requirements

The sensory requirements shall comply with the provisions of Table 1.

Appendix A

Inspection Method

A.1 General

Unless otherwise specified in this Standard, the purity of all reagents shall be above analytically pure; and the standard solutions, preparations and products used for the determination of standard titration solutions and impurities shall be prepared in accordance with the provisions of GB/T 601, GB/T 602 and GB/T 603.The water used in the experiment shall meet the requirements of Class-III water in GB/T 6682.The solution used in the test refers to the aqueous solution when the reagent is not specified.

A.2 Pre-treatment of sample A.2.1 Take at least 3 dried sea cucumbers, put them into a high-speed pulverizer for pulverization (25 000r/min, 10s/time~15s/time); and pulverize them for several times until all the specimens pass through the 830µm (20 mesh) sieve. The specimen after treatment shall be sealed and ready for use.

A.2.2 The samples processed by this method are mainly used for the detection of protein, moisture, salt and other indicators.

A.3 Rehydration of dried sea cucumbers A.3.1 Pre-soaking Take 2~3 pieces of dried sea cucumbers; weigh about 10g (m1, accurate to 0.01g); put them in a 1000mL beaker; pour in water (the amount of water is about 50 times the mass of the sea cucumber; and the sea cucumber body should be submerged); then cover the watch glass; soak at room temperature for 24h.

A.3.2 Cleaning

The sea cucumber body was cut in the soaking solution; the mud and sand attached to the sea cucumber body are cleaned; after the calcareous in the mouth is removed, and then cut into strips with a width of about 5 mm; the sea cucumber body, mud sand and calcareous in the mouth are all retained in the original soaking solution.

A.3.3 Boiling

Place the sample treated as per A.3.2 and the immersion solution in the original beaker; cover with a watch glass; boil over high heat; then reduce to low heat; keep boiling and continue to boil for 30 min; let it cool to room temperature; and place it in a 0°C~10°C refrigerator; place it for 20h. During the boiling and soaking process, the amount of water shall be kept submerging the sea cucumber body.

A.3.4 Specimen

A.3.4.1 Pour all the leaching solution and sea cucumber body treated as per A.3.3 into a 1000mL graduated cylinder, make constant volume to 600mL; and mix well.

A.3.4.2 Take out the sea cucumber and put it into a beaker; add 600mL of water; boil it again according to the method in A.3.3.Take out the sea cucumber after it has been placed in the refrigerator; absorb the surface moisture with filter paper; mince it for later use of testing the pollutants and veterinary drug residue.

A.3.4.3 Filter the soaking liquid and transfer it to ashless filter paper for the detection of sand content.

A.3.4.4 The obtained filtrate is used for the detection of water-soluble total sugar. When the sugar content in the test solution is high, it shall be diluted with water before testing; the appropriate sugar content in the test solution is 30µg/mL~70µg/mL.

A.4 Test method for dry weight ratio after rehydration in dried sea cucumber A.4.1 Principle the dried sea cucumber is rehydrated to remove various water-soluble substances from the sea cucumber; and then the dry matter mass fraction obtained by drying the sea cucumber.

A.4.2 Instruments and equipment A.4.2.1 Beaker. Tall-type, 1000mL.

A.4.2.2 Weighing flask.

A.4.2.3 Electric heating constant temperature drying oven.

A.4.2.4 Dryer. With effective desiccant.

A.4.2.5 Balance. the sensory amount is 0.1mg.

A.4.3 Analysis procedures A.4.3.1 Take the whole dried sea cucumber; weigh it (m2, accurate to 0.0001g); put it in a 1000mL beaker; pour in water (the amount of water is about 50 times the mass of the sea cucumber; and the sea cucumber body shall be submerged); cover it with watch glass; soak at room temperature for 24 h; cut the sea cucumber body; the mud and sand attached to the sea cucumber body are cleaned in the original soaking solution; and the limestone in the mouth is carefully removed.