GB 28329-2012National food safety standards -- Food additives -- Cis-3-hexenol acetate (acetic acid esters leaf) (English PDF)

1 Scope

This standard is applicable to food additives made from acetic anhydride (or acetic acid) and cis-3-hexenol as raw materials cis-3-hexenol acetate (Cellulose acetate).

2 Chemical name, molecular formula, structural formula and relative molecular mass

3 Technical requirements

3.1 sensory requirements. should be consistent with the provisions of Table 1.

Table 1 sensory requirements

The project requires a test method Color colorless to pale yellow Tissue state liquid Place the sample in a colorimetric tube with visual inspection Observation of law A strong aroma of fragrant green GB/T 14454.2

3.2 Physical and chemical indicators. should be consistent with the provisions of Table 2.

Table 2 Physical and chemical indicators

Item Index Test Method Solubility (25 ° C) 1 mL The sample was dissolved in 1 mL of 95% by volume ethanol. GB/T 14455.3 Cis-3-hexenol acetate content, w /% Cis and trans isomers >=98.0 Cis isomer = 92.0 Appendix A Acid value (in KOH)/(mg/g) <= 1.0 GB/T 14455.5 Refractive index (20 ° C) 1.425 ~ 1.429 GB/T 14454.4 Relative density (25 °C/25 °C) 0.896 ~ 0.901 GB/T 11540 Appendix A Determination of cis - 3 - hexenol acetate content A.1 Instruments and equipment A.1.1 chromatograph. according to GB/T 11538-2006 in Chapter 5 of the provisions.

A.1.2 Column. Capillary column.

A.1.3 Detector. Hydrogen flame ionization detector.

A.2 Determination method Area normalization method. according to GB/T 11538-2006 10.4 determination of content.

A.3 repeatability and result representation According to GB/T 11538-2006 11.4 implementation of the provisions.

Food additives cis-3-hexenol acetate Typical gas chromatograms (area normalization) See Appendix B.

Appendix B

Food additive cis - 3 - hexenol acetate Typical gas chromatogram (Area normalization method) B.1 Food additive cis-3-hexenol acetate Typical gas chromatogram Food additives cis-3-hexenol acetate Typical gas chromatograms are shown in Figure B.1.

Description.

1 - cis-3-hexenol acetate;

2 --- trans-3-hexenol acetate.

Figure B.1 Food additive cis-3-hexenol acetate Typical gas chromatogram

B.2 Operating conditions B.2.1 Column. capillary column, length 30m, inner diameter 0.32mm.

B.2.2 Stationary phase. (5% phenyl) methylpolysiloxane.

B.2.3 Film thickness. 0.25 µm.

B.2.4 Chromatographic furnace temperature. 160 °C constant temperature.

B.2.5 Inlet temperature. 250 ° C.

B.2.6 Detector temperature. 250 ° C.

B.2.7 Detector. Hydrogen flame ionization detector.

B.2.8 Carrier gas. nitrogen.

B.2.9 Carrier gas flow rate. about 30 mL/min.

B.2.10 Injection volume. 0.2 µL.

B.2.11 Split ratio. 1/75.