GB 23200.17-2016Food safety national standard -- Determination of Thiabendazole Residues in Fruits and Vegetables by Liquid Chromatography (English PDF)

Foreword

This standard replaces NY/T 1649-2008 "Determination of thiabendazole residues in fruits and vegetables by high performance liquid chromatography".

This standard and NY/T 1649-2008 compared to the main changes are as follows.

- the standard name has been modified to increase the national standard for food safety;

- modified in accordance with the format of the food safety standard.

- normative reference documents to increase the GB 2763 "maximum pesticide residues in food" standard;

- the preparation of the sample to increase the sampling site requirements and refine the requirements of the preparation of the sample;

- Increased precision requirements.

National standards for food safety Determination of Thiabendazole Residues in Fruits and Vegetables by Liquid Chromatography

1 Scope

This standard specifies the method for the determination of thiabendazole residues in vegetables and fruits by high performance liquid chromatography (HPLC).

This standard applies to the determination of thiabendazole residues in vegetables and fruits.

2 Normative reference documents

The following documents are indispensable for the application of this document. For dated filing applications, only the dated edition applies to this document.

For undated references, the latest edition (including all modifications) applies to this document.

GB 2763 National Standard for Food Safety - Maximum Residue Limit of Pesticides in Foodstuffs GB/T 6682 Analytical laboratory water specifications and test methods

3 Principle

Samples of thiabendazole in the sample after extraction, according to thiophanate in acidic conditions dissolved in water, alkaline conditions dissolved in the principle of ethyl acetate, Purification, and then by the reverse phase chromatography, UV detector 300nm detection, according to the retention time qualitative, external standard method quantitative.

4 Reagents and Materials

Unless otherwise stated, only reagents identified as analytical reagents and water at GB/T 6682 level were used in the analysis.

5 Instruments and equipment

5.1 High Performance Liquid Chromatograph with UV Detector.

5.2 Analysis of balance. 0.01 g and 0.1 mg.

5.3 Tissue crusher.

5.4 Rotary Evaporator.

5.5 Mechanical reciprocating oscillator.

5.6 Buchner funnel.

6 Sample preparation

Sampling samples of vegetables and fruit samples according to GB 2763-2014 Appendix A sampling, for the smaller individual samples, after sampling the whole for the individual large uniform sample can be split on the axis of symmetry or symmetry or cut into small pieces of post-processing; for slender, flat Flat or component content in the various parts of the sample can be cut in different parts of small pieces or cut into small pieces or processing; take the sample after the chopped, Fully mixed, with a quartile sampling or directly into the tissue crusher mashed into homogenate. The homogenate was placed in a polyethylene bottle at -16 ° C to -20 ° C Save under the file.

7 Analysis steps

9 Precision

The ratio of the absolute difference between the two independent determinations obtained under reproducibility and its arithmetic mean (percentage) shall be in accordance with the Record A requirements.

The ratio of the absolute difference between the two independent determinations obtained under reproducibility and its arithmetic mean (percentage) shall be in accordance with the Record B requirements.

10% limit

The limit method of this standard method is 0.05mg/kg.

11 Chromatogram.

Thiabendazole standard solution map shown in Figure 1.

Figure 1 1.0mg/L thiabendazole standard solution map shown in Figure

Appendix A

(Normative appendix) Laboratory repeatability requirements

Table A.1 Laboratory repeatability requirements

Measured component content Mg/kg Precision <=0.001 36 > 0.01 > 1 14 Appendix B (Normative appendix) Inter-laboratory reproducibility requirements

Table B.1 Inter-laboratory reproducibility requirements

Measured component content Mg/kg Precision <=0.001 54 > 0.01 > 1 19