GB 1903.10-2015National food safety standard -- Food Nutrition Enhancer -- Ferrous gluconate (English PDF)

2.1 Chemical Name

D- glucose di ferric salt Formula 2.2 C12H22FeO14 · nH2O (n = 0 or 2)

2.3 Formula

2.4 Relative molecular mass

446.15 (anhydrous) (according to 2012 international relative atomic mass) 482.18 (dihydrate) (according to 2012 international relative atomic mass)

3.1 Sensory requirements

Sensory requirements shall comply with the requirements of Table 1.

Table 1 Sensory requirements

Project requires test methods Greyish yellow or light yellow green color Status crystalline powder or granules Smell of caramel-like odor Take the right amount of sample is placed in a white clean, dry vessel, the concept in natural light Examine its color, state, smell the smell

3.2 Physical and Chemical Indicators

Physical and chemical indicators should be consistent with the provisions of Table 2.

Table 2. Physical and chemical indicators

Item Index Test Method Ferrous gluconate (C12H22FeO14) content (Dry basis), w /% 97.0 102.0 Appendix A A.3 Loss on drying, w /% <= 10.0 GB 5009.3a direct drying Ferric iron (Fe3 to count), w /% <= 2.0 Appendix A A.4 by reducing sugar test A.5 in Appendix A Sulfate (SO4 dollars), w /% <= 0.1 A.6 in Appendix A Lead (Pb)/(mg/kg) <= 2.0 GB 5009.12 Total arsenic (As)/(mg/kg) <= 2.0 GB 5009.11 a drying temperature of 105 °C ± 2 °C, drying time of 16h.

Appendix A

Testing method

A.1 General Provisions this standard reagents and water, did not indicate when the other requirements, refer to the three water analytical reagent and GB/T 6682 stipulated.

Standard solutions used in the tests, the determination of impurities standard solution preparation and its products, did not indicate when the other requirements, according to GB/T 601, GB/T 602, the provisions of the preparation of GB/T 603's. Solution was used in the tests did not indicate what is formulated with solvent, it refers to an aqueous solution.

A.2 Identification Test A.2.1 Reagents and materials A.2.1.1 glacial acetic acid.

A.2.1.2 ethanol.

A.2.1.3 phenylhydrazine.

A.2.1.4 ferricyanide solution. 100g/L, prepared just before use.

A.2.2 Identification method A.2.2.1 solubility Soluble in water, almost insoluble in alcohol.

A.2.2.2 Identification of ferrous ions Weigh about 0.5g sample was dissolved in 100mL of water, a solution of potassium ferricyanide solution, generating a dark blue precipitate.

A.2.2.3 Identification of glucose A.2.2.3.1 Method summary Samples in acetic acid medium, and phenylhydrazine were hot, a yellow crystalline glucose acid phenylhydrazine.

A.2.2.3.2 analysis step Weigh a sample of about 0.5g, add 10mL water, was dissolved (heating if necessary), to take 5mL, adding 1mL 0.7mL glacial acetic acid and freshly distilled benzene Hydrazine in a water bath heated 30min, to room temperature, the inner wall of the tube with a glass rod friction, the precipitated yellow crystals.

A.3 Determination of ferrous gluconate (C12H22FeO14) content (dry basis) of A.3.1 Method summary in acidic medium, titration with cerium sulfate standard solution titration to 1,10-phenanthroline indicator solution indicates the end.

A.3.2 Reagents and materials A.3.2.1 zinc.

A.3.2.2 sulfuric acid.

A.3.2.3 sulfuric acid solution. c ( 2H2SO4 ) = 2mol/L. Amount of sulfuric acid 60mL, slowly inject 1000mL water, cooling and shake.

A.3.2.4 cerium sulfate standard titration solution. c (CeSO4) = 0.1mol/L.

A.3.2.5 1,10- phenanthroline indicator solution. Weigh 0.5g ferrous sulphate, add 100mL water dissolved, add 2 drops of sulfuric acid solution and 0.5g 1,10-phenanthroline, shake the solution stored in a sealed container.

A.3.3 Analysis step

Weigh about 1.5g sample, accurate to 0.0001g, placed in 500mL conical flask, add 75mL of water and 15mL sulfuric acid solution to dissolve, Plus 250mg of zinc dust until the solution bleaching, in order to cover a small beaker Erlenmeyer flask, set at room temperature for 20min. The surface covered with a thin layer of zinc powder Sintered glass filter funnel (or vacuum filtration), and 10mL to 10mL aqueous sulfuric acid solution were washed with a sintered glass funnel, and the combined filtrate Washing liquid to 1,10-phenanthroline indicator solution as an indicator, the rapid titration with cerium sulfate standard titration solution until the solution color changed from yellow to orange green. At the same time a blank test.

A.3.4 Calculation Results Ferrous gluconate (C12H22FeO14) content (dry basis) of the mass fraction w1, according to equation (A.1) Calculated.

V1-V0

1000 × c1 × M1

m1 × (1-w2) ×

100% (A.1)

Where.

V1 --- cerium sulfate standard titration volume of the sample solution titration solution consumed in milliliters (mL);

V0 --- titrate blank solution volume consumed cerium sulfate standard titration solution, in milliliters (mL);

1000 --- conversion factor;

Concentration c1 --- cerium sulfate standard titration solution, expressed in moles per liter (mol/L);

Molar mass M1 --- ferrous gluconate, in units of grams per mole (g/mol), [M1 (C12H22FeO14) = 446.15];

M1 --- the quality of the sample, in grams (g);

w2 --- Found loss on drying of the sample mass fraction,%.

Parallel determination results take twice the arithmetic mean prevail. Two parallel determination results is not more than 0.3% absolute difference.

A.4 ferric iron (Fe3 in dollars) Determination A.4.1 Reagents and materials A.4.1.1 potassium iodide.

A.4.1.2 hydrochloric acid.

A.4.1.3 sodium thiosulfate standard titration solution. c (Na2S2O3) = 0.1mol/L.

A.4.1.4 starch indicator solution. 10g/L.

A.4.2 Analysis step

Weigh 5g samples, accurate to 0.0001g, set iodine bottle, add water 100mL, 10mL dissolved in hydrochloric acid, potassium iodide was added 3g, Seal stopper and shake to dissolve, in the dark for about 5min, plug and play with a little water rinse lids, add starch indicator solution 0.5mL, promptly Sodium thiosulfate standard titration solution titrated to a solution blue color disappears as the end point, that is, too. At the same time a blank test.

A.4.3 Calculation Results Ferric iron (Fe3 to count) mass fraction w3, according to equation (A.2) Calculated.

V3-V2

1000 × c2 × M2

100% (A.2)

Where.

Volume of sodium thiosulfate standard titration V3 --- titration solution consumed by the sample solution, in milliliters (mL);

V2 --- titrate blank solution volume consumed by sodium thiosulfate standard titration solution, in milliliters (mL);

1000 --- conversion factor;

Concentration c2 --- sodium thiosulfate standard titration solution, expressed in moles per liter (mol/L);

--- The M2 iron molar mass in grams per mole (g/mol), [M2 (Fe) = 55.85];

m2 --- sample mass, in grams (g).

Parallel determination results take twice the arithmetic mean prevail. Two parallel determination results is not more than 0.1% absolute difference.

A.5 Determination of reducing sugar A.5.1 Reagents and materials A.5.1.1 anhydrous sodium carbonate (Na2CO3).

A.5.1.2 hydrogen sulfide gas. Take a small amount of pyrite powder, add appropriate amount of dilute hydrochloric acid, hydrogen sulfide gas is exported.

A.5.1.3 hydrochloric acid. 36%.

A.5.1.4 ammonia. 25%.

A.5.1.5 Fehling solution.

A.5.1.6 hydrochloric acid solution. 3mol/L. Take 258mL36% hydrochloric acid, diluted with water to 1L.

A.5.1.7 ammonia solution. 6mol/L. Take 450mL25% aqueous ammonia diluted with water to 1L.

A.5.1.8 sodium carbonate solution. 10.5%. Weigh 10.5g anhydrous sodium carbonate (Na2CO3), add 89.5g dissolved in water.

A.5.1.9 lead acetate paper.

A.5.2 Analysis step

Weigh 0.5g sample, add water to dissolve 10mL, warm, ammonia solution and the solution is added 1mL alkaline hydrogen sulfide gas from the precipitation Promoter, was allowed to stand 30min, filtered, washed twice with 10mL water, filtrate and washings were combined, acidified with hydrochloric acid, hydrochloric acid solution was added 2mL Liquid, boiling until the steam not to wet lead acetate paper black, the solution was continued to boil a volume of about 10mL, was cooled, was added 5mL carbon Sodium and 20mL water, filtered, and the filtrate was diluted with water to 100mL. Draw solution 5mL, added 2mL Fehling solution, boiled 1min should not produce a red precipitate, namely through tests.

A.6 Sulfate (SO4 meter) measurement A.6.1 Reagents and materials A.6.1.1 hydrochloric acid solution. 13.

A.6.1.2 barium chloride solution. 250g/L.

A.6.1.3 Potassium standard solution. 0.1mg/mL.

A.6.2 Analysis step

Weigh 1.0g sample to the nearest 0.01g, add 40mL of water to dissolve, the solution was adjusted to neutral with hydrochloric acid solution, diluted to 50mL, Shaken, filtered, and the filtrate was retained. Take 25mL filtrate as the sample solution, placed in 50mL Nessler colorimetric tube, add 2mL of hydrochloric acid solution, Add water to a volume of about 40mL, 5mL plus barium chloride solution, dilute with water to 50mL, shake, place 10min, black background, axial Observation, the sample solution turbidity should not exceed the standard turbidity solution was.

Turbidity standard solution. Measure 5.0mL potassium sulfate standard solution, placed in 50mL colorimetric tube. And the sample solution while the same treatment.

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