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GB 1886.318-2021National food safety standard - Food additives - Zeaxanthin (English PDF)

食品安全国家标准食品添加剂玉米黄

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Issued by

NHC; SAMR

Level / Type

National · Mandatory

Issue date

February 22, 2021

Implementation date

August 22, 2021

Scope

GB 1886.318-2021 is the English-translated version of 食品安全国家标准食品添加剂玉米黄.

GB 1886.318-2021 is the Chinese national food safety standard for the food additive zeaxanthin, prepared from corn by extraction and concentration with vegetable oil as the extraction solvent.

It gives the molecular formula C40H56O2 and the relative molecular mass 568.87, the structural formula, and the sensory and physico-chemical requirements the additive has to meet.

Appendix A carries the inspection methods: identification through the absorption maximum at 455 nm plus or minus 10 nm in chloroform, and determination of the colour value with an ultraviolet-visible spectrophotometer on a specimen solution read in a 1 cm cuvette.

Appendix B reproduces the liquid chromatograms of the zeaxanthin, cryptoxanthin and lutein standard solutions. It was issued on 22 February 2021 and has applied since 22 August 2021.

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Document preview — GB 1886.318-2021

National Standard of the People's Republic of China

Issued by: NHC; SAMR

1 Scope3
2 Chemical Name of Main Components, Molecular Formula, Structural Formula and Relative Molecular Mass3
3 Technical Requirements3
Appendix A Inspection Methods5
Appendix B Liquid Reference Chromatograms of Zeaxanthin, Cryptoxanthin and Lutein Standard Solutions9

1 Scope

This Standard is applicable to corn and/or food additive of zeaxanthin that takes the corn as raw materials, and is prepared through extracting and concentrating the extraction solvents of vegetable oil.

2 Chemical Name of Main Components, Molecular Formula, Structural Formula and Relative Molecular Mass

2.1 Molecular formula

Zeaxanthin: C40H56O2

2.2 Structural formula

2.3 Relative molecular mass

Zeaxanthin: 568.87 (as per 2016 international relative atomic mass)

3 Technical Requirements

3.1 Sensory requirements

The sensory requirements shall comply with the provisions of Table 1.

Appendix A Inspection Methods

A.1 General provisions

If no other requirements are specified, all the reagents and water used in this Standard refer to the analytical reagents and Class-III water specified in GB/T 6682. If no other requirements are specified, all the standard solution used in the test, standard solution for determining the impurity, preparation and product shall be prepared according to the provisions of GB/T 601, GB/T 602, GB/T 603. The solution used in the test refers to the aqueous solution when the solvent is not specified.

A.2 Identification test

Take an appropriate amount of specimen and dissolve it in chloroform. The specimen solution shall have a maximum absorption peak in the wavelength range of 455nm ± 10nm.

A.3 Determination of colour value

A.3.1 Reagents and materials

Chloroform.

A.3.2 Apparatus

Ultraviolet-visible spectrophotometer.

A.3.3 Analysis procedures

Accurately weigh 0.1g of specimen (accurate to 0.0001g), dissolve it by chloroform; transfer to a 100mL volumetric flask; add chloroform to make constant volume to the mark; and shake well. Use this solution as a specimen solution; place it in a 1cm cuvette; use chloroform as a blank control; and use an ultraviolet-visible spectrophotometer to measure the absorbance (the absorbance shall be controlled at 0.3~ 0.7; otherwise, the concentration of the specimen solution shall be adjusted; and then the absorbance shall be re-measured) at the maximum absorption wavelength of 455nm±10nm.

A.3.4 Calculation of results

The colour value E1% 1cm (455nm±10nm) is calculated according to Formula (A.1). […]

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 16 pages — is available in the English PDF.

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