GB 1886.318-2021National food safety standard - Food additives - Zeaxanthin (English PDF)

1 Scope

This Standard is applicable to corn and/or food additive of zeaxanthin that takes the corn as raw materials, and is prepared through extracting and concentrating the extraction solvents of vegetable oil.

2 Chemical Name of Main Components, Molecular

Formula, Structural Formula and Relative Molecular Mass

3 Technical Requirements

Appendix A Inspection Methods

A.1 General provisions If no other requirements are specified, all the reagents and water used in this Standard refer to the analytical reagents and Class-III water specified in GB/T 6682. If no other requirements are specified, all the standard solution used in the test, standard solution for determining the impurity, preparation and product shall be prepared according to the provisions of GB/T 601, GB/T 602, GB/T 603.The solution used in the test refers to the aqueous solution when the solvent is not specified.

A.2 Identification test Take an appropriate amount of specimen and dissolve it in chloroform. The specimen solution shall have a maximum absorption peak in the wavelength range of 455nm +/- 10nm.

A.3 Determination of colour value A.3.1 Reagents and materials Chloroform.

A.3.2 Apparatus Ultraviolet-visible spectrophotometer.

A.3.3 Analysis procedures Accurately weigh 0.1g of specimen (accurate to 0.0001g), dissolve it by chloroform;

transfer to a 100mL volumetric flask; add chloroform to make constant volume to the mark; and shake well. Use this solution as a specimen solution; place it in a 1cm cuvette; use chloroform as a blank control; and use an ultraviolet-visible spectrophotometer to measure the absorbance (the absorbance shall be controlled at 0.3~ 0.7; otherwise, the concentration of the specimen solution shall be adjusted; and then the absorbance shall be re-measured) at the maximum absorption wavelength of 455nm+/-10nm.

A.3.4 Calculation of results The colour value E1% 1cm (455nm+/-10nm) is calculated according to Formula (A.1).