GB 1886.295-2016National Food Safety Standard -- Food Additives - 2, 3, 5, 6-tetramethyl pyrazine (English PDF)

2.1 Chemical Name

2,3,5,6-methylpyrazine Formula 2.2 C8H12N2

2.3 Formula

2.4 Molecular Weight

136.20 (according to 2007 international relative atomic mass)

3.1 Sensory requirements

Sensory requirements shall comply with the requirements in Table 1.

Table 1 Sensory requirements

Project requires test methods Color status white Crystal or powder the sample is placed on a clean white paper, were visually Observed Musty aroma, flavor and fermentation coffee-like aroma GB/T 14454.2

3.2 Physical and Chemical Indicators

Physical and chemical indicators should be consistent with the provisions of Table 2.

Table 2. Physical and chemical indicators

Item Index Test Method 2,3,5,6-tetramethyl-pyrazine content, w /% >= 98.0 Appendix A Melting point/°C 85.0 ~ 90.0 GB/T 14457.3 Appendix A Determination of the content of 2,3,5,6-tetramethyl pyrazine A.1 instruments and equipment A.1.1 Chromatography. in GB/T 11538-2006 specified in Chapter 5.

A.1.2 Column. capillary column.

A.1.3 Detector. hydrogen flame ionization detector.

A.2 Determination

Area normalization method. Press the GB/T 11538-2006 Determination 10.4.

Sample Preparation. Weigh 1g of this product was dissolved in 10 mL of 95% (volume fraction) of ethanol, shake up.

A.3 repeatability and results are expressed 11538-2006 carried out in accordance with the provisions of 11.4 GB/T , should meet the requirements.

2,3,5,6-tetramethyl pyrazine food additive gas chromatogram and operating conditions see Appendix B.

Appendix B

Food additive 2,3,5,6-tetramethyl pyrazine GC operating conditions and FIG.

(Area normalization method) B.1 food additive 2,3,5,6-tetramethyl pyrazine GC FIG.

2,3,5,6-tetramethyl pyrazine food additive gas chromatography is shown in Figure B.1.

Description.

1 --- Ethanol (solvent);

2 --- 2,3,5,6-tetramethylpyrazine.

Figure B.1 food additive 2,3,5,6-tetramethyl pyrazine GC FIG.

B.2 operating conditions B.2.1 Column. capillary column, length 30m, internal diameter 0.25mm.

B.2.2 stationary phase. polyethylene glycol 20000.

B.2.3 film thickness. 0.25µm.

B.2.4 chromatographic oven temperature. 140 °C thermostat 10min, then linear temperature programmed from 140 deg.] C to 200 °C, a rate of 10 °C/min, and finally 200 °C thermostat 10min.

B.2.5 Inlet temperature. 250 °C.

B.2.6 detector temperature. 250 °C.

B.2.7 Detector. hydrogen flame ionization detector.

B.2.8 Carrier gas. nitrogen.

B.2.9 carrier gas flow rate. head pressure 60kPa.

B.2.10 Injection volume. about 2µL.

B.2.11 split ratio. 100.1.