GB 1886.233-2016National food safety standard - Food additives - Vitamin E (English PDF)

2.1 Chemical name

2.2 Molecular formula

2.3 structural formula

For d-alpha-tocopherol, dl-alpha-tocopherol, d-alpha-tocopheryl acetate, dl-alpha-tocopheryl acetate,

d-alpha-tocopheryl acid succinate, dl-alpha-tocopheryl acid succinate, d-alpha-tocopheryl acetate

concentrate and mixed tocopherols concentrate, the R1 group, R2 group, R3 group and

R4 group corresponding to their structural formula are shown as Table 1.

2.4 Relative molecular mass

d-alpha-tocopherol. 430.71 (according to the international relative atomic mass in 2013);

dl-alpha-tocopherol. 430.71 (according to the international relative atomic mass in 2013);

d-alpha-tocopheryl acetate. 472.75 (according to the international relative atomic mass in

2013);

3.1 Sensory requirements

Sensory requirements should be in accordance with Table 2.

Appendix A

Test Method

A.1 General provisions

Unless otherwise specified in this standard, the purity of reagents used shall be above

the grade of analytical reagent; the used standard titration solutions, the standard

solutions for the determination of impurities, the preparations and products shall be

prepared in accordance with the provisions of GB/T 601, GB/T 602 and GB/T 603; the

test water should meet the requirements of grade-3 water specified by GB/T 6682.

The solution used in the test, if not indicated which solvent is used, refers to aqueous

solution.

A.2 Identification test

A.2.1 Reagents and materials

A.2.1.1 Absolute ethanol.

A.2.1.2 Nitric acid.

A.2.2 Identification method

A.3 Content determination

A.3.1 Reagents and materials

A.3.1.1 Cetyl palmitate.

A.3.1.2 alpha-tocopherol reference substance.

A.3.1.3 alpha-tocopheryl acetate reference substance.

A.3.1.4 alpha-tocopheryl acid succinate reference substance.

A.3.1.5 n-hexane.

A.3.2 Instruments and apparatuses

Gas chromatography.

A.3.3 Analysis steps

A.3.3.1 Determination of d-alpha-tocopherol content

A.3.3.1.2 Reference chromatographic conditions

SE-30 capillary column; hydrogen flame ion detector; keep the oven temperature at

250°C; the vaporization temperature and detection temperature are 300°C; the carrier

gas is nitrogen; adjust the carrier gas flow rate to make the internal standard peak

retention time at 18 min ~ 20 min. Inject 1 µL of the reference solution into the

chromatograph; record the chromatogram.

A.3.3.1.3 Correction factor determination

Inject 1 µL of reference solution into the gas chromatograph; record the chromatogram;

measure Aref1, the peak area of the alpha-tocopherol, and Aint1, the peak area of the internal

standard peak; calculate the correction factor f1 according to Formula (A.1).

A.3.3.1.4 Determination

Inject 1 µL of the sample solution into the gas chromatograph; record the

chromatogram; calculate the peak area of alpha-tocopherol, beta- and gamma-tocopherol, and delta-

tocopherol (Aalpha2, Abeta,gamma2, Adelta2); calculate the sum of the first three items (At2) and the

internal standard peak area Aint2; calculate the content according to Formula (A.2) and

Formula (A.3).

A.3.3.2.1 Solution preparation

The internal standard solution and the reference solution are the same as A.3.3.1.1.

Sample solution. weigh an appropriate amount of sample; accurate to 0.02 mg; place

it in a brown volumetric flask; add internal standard solution to dilute to 10.0 mL; shake

to dissolve to gain a solution containing d-beta-+d-gamma-tocopherol of about 1.5 mg/mL.

A.3.3.2.2 Reference chromatographic conditions

Same as A.3.3.1.2.

A.3.3.2.3 Correction factor determination

Same as A.3.3.1.3.

A.3.3.3.4 Determination

Inject 1 µL of sample solution into the gas chromatograph; record the chromatogram.

Calculate Asample7, the peak area of the alpha-tocopheryl acetate, and Aint7, the peak area

of the internal standard; calculate the content according to Formula (A.7).

A.6 Determination of absorbance coefficient

A.6.1 Reagents and materials

Absolute ethanol.

A.6.2 Instruments and apparatuses

Spectrophotometry.

A.6.3 Analysis steps

Weigh about 0.02 g of sample; accurate to 0.002 g; add absolute ethanol and dilute to