GB 1886.195-2016National Food Safety Standard -- Food Additives -- Isoamyl Butyrate (English PDF)

2.1 Chemical name

3-methylbutyl butyrate, 2-methylbutyl butyrate

2.2 Molecular formula

C9H18O2

2.3 Structural formula

Butyric acid 3-methylbutyl butyrate 2-methylbutyl ester

2.4 Relative molecular mass

158.24 (according to the 2007 International Relative Atomic Quality)

3.1 Sensory requirements

Sensory requirements shall comply with the requirements of Table 1.

Table 1 sensory requirements

The project requires a test method Color and colorless State liquid the sample was placed in a colorimetric tube and visually observed Aroma aroma aroma GB/T 14454.2

3.2 Physical and chemical indicators

Physical and chemical indicators should be consistent with the provisions of Table 2.

Table 2 Physical and chemical indicators

Item Index Test Method Solubility (25 ° C) 1 mL The sample is fully dissolved in 4 mL of 70% by volume ethanol. GB/T 14455.3 Butyrate content, w /% >= 98.0 (sum of two isomers) Appendix A Acid value (in KOH)/(mg/g) <= 1.0 GB/T 14455.5 Refractive index (20 ° C) 1.409 ~ 1.414 GB/T 14454.4 Relative density (25 °C/25 °C) 0.861 ~ 0.866 GB/T 11540 Appendix A Determination of Isoamyl Alcohol Content A.1 Instruments and equipment A.1.1 chromatograph. according to GB/T 11538-2006 in Chapter 5 of the provisions.

A.1.2 Column. Capillary column.

A.1.3 Detector. Hydrogen flame ionization detector.

A.2 Determination method Area normalization method. according to GB/T 11538-2006 10.4 determination of content.

A.3 repeatability and result representation According to GB/T 11538-2006 11.4 provisions, should meet the requirements.

Food additives and isoamyl butyrate gas chromatograms and operating conditions see Appendix B.

Appendix B

Food additive - Isoamyl butyrate gas chromatogram and operating conditions (Area normalization method) B.1 food additive isopentyl butyrate gas chromatogram Food additives Butyl amyl butyrate gas chromatogram shown in Figure B.1.

Description.

1 - Butyric acid 3 - methyl butyl ester;

2 - butyric acid 2 - methyl butyl ester.

Figure B.1 Food additive Isoamyl butyrate gas chromatogram

B.2 Operating conditions B.2.1 Column. capillary column, length 30m, inner diameter 0.32mm.

B.2.2 Stationary phase. 100% dimethylpolysiloxane.

B.2.3 Film thickness. 0.25 µm.

B.2.4 Chromatographic furnace temperature. linear program temperature from 50 °C to 220 °C, the rate of 3 °C/min.

B.2.5 Inlet temperature. 280 ° C.

B.2.6 Detector temperature. 280 ° C.

B.2.7 Detector. Hydrogen flame ionization detector.

B.2.8 Carrier gas. nitrogen.

B.2.9 Pre-column pressure. 45 kPa.

B.2.10 Injection volume. 0.1 µL.

B.2.11 Split ratio. 100. 1.