GB 1886.138-2015National Food Safety Standard -- Food Additives - 2-acetyl pyrazine (English PDF)

2.1 Chemical name

2-acetylpyrazine

2.2 Molecular formula

C6H6N2O

2.3 Structural formula

2.4 Relative molecular mass

122.13 (according to the 2007 International Relative Atomic Quality)

3.1 Sensory requirements

Sensory requirements shall comply with the requirements of Table 1.

Table 1 sensory requirements

The project requires a test method Colorless to pale yellow State of crystallization Place the sample on a clean white paper and observe it visually Aroma with the aroma of popcorn

3.2 Physical and chemical indicators

Physical and chemical indicators should be consistent with the provisions of Table 2.

Table 2 Physical and chemical indicators

Item Index Test Method Solubility (25 ° C) 1 g Samples are fully dissolved in 20 mL 95% (volume fraction) Ethanol GB/T 14455.3 2-acetylpyrazine content, w /% >= 99.0 Appendix A Melting point/°C 76.0 ~ 78.0 GB/T 14457.3 Appendix A Determination of 2 - acetylpyrazine content A.1 Instruments and equipment A.1.1 chromatograph. according to GB/T 11538-2006 in Chapter 5 of the provisions.

A.1.2 Column. Capillary column.

A.1.3 Detector. Hydrogen flame ionization detector.

A.2 Determination method Preparation of the sample. Weigh the sample 1g dissolved in 10mL 95% (volume fraction) of ethanol, shake back.

Area normalization method. according to GB/T 11538-2006 10.4 determination of content.

A.3 repeatability and result representation According to GB/T 11538-2006 11.4 provisions.

Food additive 2-acetylpyrazine gas chromatogram (area normalization) See Appendix B.

Appendix B

Food Additive 2 - Acetylpyrazine Gas Chromatography (Area normalization method) B.1 Food Additive 2-Acetylpyrazine Gas Chromatography the food additive 2-acetylpyrazine gas chromatogram is shown in Figure B.1.

Description.

1 --- Ethanol (solvent);

2 --- 2-acetylpyrazine.

Figure B.1 Gas Chromatography of 2-Acetylpyrazine Food Additives

B.2 Operating conditions B.2.1 Column. capillary column, length 30m, inner diameter 0.25mm.

B.2.2 Stationary phase. Polyethylene glycol.

B.2.3 Film thickness. 0.25 µm.

B.2.4 Chromatographic furnace temperature. linear program temperature from 150 °C ~.200 °C, the rate of 10 °C/min.

B.2.5 Inlet temperature. 240 ° C.

B.2.6 Detector temperature. 240 ° C.

B.2.7 Detector. Hydrogen flame ionization detector.

B.2.8 Carrier gas. nitrogen.

B.2.9 Pre-column pressure. 0.07 MPa.

B.2.10 Injection volume. 2 µL.

B.2.11 Split ratio. 100. 1.