GB 15193.19-2015National food safety standard -- Methods for disposing mutagens, teratogens and carcinogens (English PDF)

3.1 Benzidine, beta- naphthylamine

3.1.1 Reagents

2mol/L sulfuric acid, 0.2mol/L potassium permanganate, sodium metabisulfite, 10mol/L of potassium hydroxide.

3.1.2 Processing Method

3.1.2.1 such as benzidine or beta- naphthylamine concentrations higher than 0.9mg/mL, diluted with water to 0.9mg/mL or less.

3.1.2.2 Each 10mL benzidine or beta- naphthylamine was added 5mL0.2mol L potassium permanganate solution/and 5mL2mol/L sulfuric acid solution Liquid, the reaction should be continued for at least 10h.

3.1.2.3 Each 10mL reaction system was added 0.8g of sodium metabisulfite decolorization of the reaction system, if not completely decolorized then add more.

3.1.2.4 Each reaction system mentioned above was added 10mL 8mL10mol/L of potassium hydroxide, the reaction system is strongly alkaline (pH > 12), the trans should exothermic process.

3.1.2.5 plus diluted with 100mL of water, filtered manganese compound, and the filtrate after the chemical waste in accordance with waste disposal.

Sodium azide 3.2

3.2.1 Reagents

Sodium nitrite, 4mol/L of sulfuric acid, the volume ratio of mass concentration of 10% potassium iodide solution, 1mol/L hydrochloric acid, starch.

3.2.2 Solution preparation

Sodium nitrite solution. 7.5g of sodium nitrite in 38mL water.

3.2.3 Processing Method

3.2.3.1 Each 5g of sodium azide were dissolved in 100mL of water.

3.2.3.2 sodium nitrite solution with stirring to a solution of sodium azide (3.2.3.1) in.

3.2.3.3 slowly added 4mol/L sulfuric acid until acidic reaction system and the stirring was continued for 1h, such as more samples, the process proceeds with an ice bath Carried out this operation need to be carried out in a fume hood.

3.2.3.4 should be sodium nitrite, and then adding sulfuric acid, was added in reverse order will produce harmful substances.

3.2.3.5 Add a few drops of the reaction solution to a system of equal volume mass concentration of 10% by volume potassium iodide solution was added 1 drop of 1mol/L Hydrochloric acid and 1 drop of the starch solution. If the reaction solution dark blue shows the presence of excess nitrite that the reaction has been completed, in accordance with the waste chemical waste disposal Management. If you are not adding more excess sodium nitrite.

3.3 Aflatoxin B1

3.3.1 Reagents

5.25% sodium hypochlorite, acetone.

3.3.2 Processing Method

3.3.2.1 mg of aflatoxin per 2mL5.25% sodium hypochlorite solution was added, the reaction system was allowed to stand overnight, sodium hypochlorite solution at any time Room slow decomposition, should be detected in the active chlorine content of the solution at regular intervals.

3.3.2.2 After addition of 3 volumes of water and diluted with a total volume equivalent to 5% of acetone, the reaction of at least 30min. After the process is completed in accordance with waste Chemical waste.

3.4 PAHs (benzo [a] pyrene, 7,12-dimethyl-benz [a] anthracene, 3-methyl gall anthracene, 2-amino-fluorene, 2,7-diamino fluorene, 2-acetamido Fluorene, benz [a] anthracene, 2-nitrofluorene, 9,10-dimethyl anthracene, trinitro fluorenone)

3.4.1 Reagents

Potassium permanganate, sulfuric acid, sodium metabisulfite, 10mol/L of potassium hydroxide and acetone.

3.4.2 Preparation of sulfuric acid solution of potassium permanganate

Added 1L3mol/L sulfuric acid in 47.4g of potassium permanganate, stirring 15min ~ 60min backup, using now.

3.4.3 Processing Method

3.4.3.1 Each 5mg PAHs added 2mL acetone and ensure that polycyclic aromatic hydrocarbons completely dissolved.

3.4.3.2 Each 5mg PAHs added 10mL sulfuric acid solution of potassium permanganate, the reaction system should be stirred for 1h. The need to maintain throughout the reaction the reaction system was purple, if not more potassium permanganate solution of sulfuric acid should be added until the reaction system was kept above purple 1h.

3.4.3.3 Each 10mL reaction system was added 0.8g of sodium metabisulfite decolorization of the reaction system, if not completely decolorized then add more.

3.4.3.4 Each reaction system was added 10mL 8mL10mol/L of potassium hydroxide, the reaction system is strongly alkaline (pH > 12), through the reaction Exothermic process.

3.4.3.5 plus diluted with 100mL of water, filtered manganese compound, and the filtrate after the chemical waste in accordance with waste disposal.

3.4.3.6 such as polycyclic aromatic hydrocarbons less than 5mg, should also be addressed in accordance with the method 3.4.3.1 ~ 3.4.3.5.

3.5 N- methyl -N- -N- nitro-nitrosoguanidine, N- nitroso-methyl urea, Ethylnitrosourea

3.5.1 Reagents

Methanol, 6mol/L hydrochloric acid, sulfamic acid.

3.5.2 Processing Method

3.5.2.1 Each 30g methyl - nitro - nitrosoguanidine, N- nitroso-methyl urea or Ethylnitrosourea added 1L of methanol.

3.5.2.2 added slowly with stirring and 1L6mol/L hydrochloric acid.

3.5.2.3 sulfamic acid 70g was added, the reaction system was stirred for at least 24h. Waste water treatment in accordance with the chemical waste after processing is completed.

3.6 Mitomycin C

3.6.1 Reagents

5.25% sodium hypochlorite, 1% sodium hydrogen sulfite.

3.6.2 Processing Method

3.6.2.1 As the concentration is too high, it should be diluted mitomycin C to not more than 0.5mg/mL.

3.6.2.2 Each 10mL mitomycin C was added 15mL5.25% sodium hypochlorite solution, the reaction process is very fast.

3.6.2.3 After the completion of the reaction was added excess 1% sodium bisulfite to remove the excess sodium hypochlorite. After the process is completed in accordance with the waste chemical waste deal with.

3.7 N- NDMA

3.7.1 Reagents

Methanol, 1mol/L of potassium hydroxide, an aluminum-nickel alloy.

3.7.2 Processing Method

3.7.2.1 The water (or methanol) N- nitroso dimethylamine was dissolved to a concentration of not more than 10mg/mL.

1mol/L potassium hydroxide solution was added an equal volume of 3.7.2.2 and using a magnetic stirrer.

3.7.2.3 Each 100mL reaction solution was slowly added 5g of aluminum-nickel alloy, should prevent excessive foaming, this exothermic reaction should be less than the total volume of container Reactor volume third.

3.7.2.4 reaction system was closed and stirring was continued for 24h.

Discard 3.7.2.5 spent nickel can be put in dry 24h metal bracket (away from combustible materials) later. After the process is completed in accordance with the waste chemical waste deal with.

3.8 Methyl methanesulfonate, ethyl methanesulfonate

3.8.1 Reagents

1mol/L NaOH.

3.8.2 Processing Method

Each compound was added 1mL 50mL1mol/L sodium hydroxide solution, the reaction system should methyl methanesulfonate was stirred 6h, ethyl methanesulfonate 48h. Waste water treatment in accordance with the chemical waste after processing is completed.

3.9 Ochratoxin A

3.9.1 Reagents

Sodium hypochlorite, ethanol.

3.9.2 Solution preparation

Sodium hypochlorite solution. 100mL5.25% sodium hypochlorite was added 200mL of water into the preparation of the appropriate concentration of sodium hypochlorite solution prepared Use, using now.

3.9.3 Processing Method

3.9.3.1 Each 1mg ochratoxin A was dissolved in 1mL ethanol and 1mL each dissolved in ethanol ochratoxin A was added 50mL times Chlorite solution.

3.9.3.2 using sonication to make it fully dissolved, the system should reflect at least 30min. After the process is completed in accordance with the waste chemical waste deal with.

3.10 heterocyclic aromatics (4-nitroquinoline-oxide -N-, ICR-170, ICR-191, triethylene melamine, furan sugar amide, 9-amino acridine, and more Polychlorinated biphenyls)

3.10.1 Reagent

Acetonitrile, sulfuric acid, potassium permanganate, sodium metabisulfite, 10mol/L of sodium hydroxide.

3.10.2 Sulfuric acid solution of potassium permanganate preparation

Added 1L3mol/L sulfuric acid in 47.4g of potassium permanganate, stirring 15min ~ 60min backup, using now.

3.10.3 Processing Method

3.10.3.1 heterocyclic aromatic per 5mg added 3mL of acetonitrile and make sure it is completely dissolved.

3.10.3.2 Each 5mg heterocyclic aromatic sulfuric acid was added potassium permanganate solution 10mL, and the reaction was stirred for 1h; during the reaction of the system should be maintained Purple, such as color fade should continue adding potassium permanganate solution of sulfuric acid, the reaction system was kept until the purple 1h more.

3.10.3.3 Each 10mL reaction system was added 0.8g of sodium metabisulfite decolorization of the reaction system, if not completely decolorized then add more.

Each reaction system was added 10mL 3.10.3.4 8mL10mol/L of potassium hydroxide, the reaction system is strongly alkaline (pH > 12), through the reaction Exothermic process.

3.10.3.5 add 100mL diluted with water, filtered manganese compound, and the filtrate after the chemical waste in accordance with waste disposal.

3.10.3.6 heterocyclic aromatics such as less than 5mg, should also be addressed in accordance with 3.10.3.1 ~ 3.10.3.5 methods.

3.11 Cyclophosphamide

3.11.1 Reagent

1mol/L hydrochloric acid, sodium thiosulfate, sodium hydroxide.

3.11.2 Processing Method

3.11.2.1 added per 250mg cyclophosphamide 10mL1mol/L hydrochloric acid, after cooling to room temperature was heated at reflux for 1h.

3.11.2.2 Add 0.2g/mL sodium hydroxide solution to pH 6, was cooled to room temperature.

3.11.2.3 per 250mg cyclophosphamide 1.5g sodium thiosulfate and 0.2g/mL of sodium hydroxide solution is strongly alkaline, the system should Reaction of at least 1h, diluted with water after completion of the reaction discarded.

3.12 Congo red

3.12.1 Reagent

AmberliteXAD-16 resin.

3.12.2 Processing Method

The 1gAmberliteXAD-16 resin was added 20mL of Congo Red concentration 100µg/mL solution and stirred for at least 2h.

Waste and chemical waste in accordance with the resin treatment after treatment is completed.

3.13 Dimethylaminobenzaldehyde diazonium sulfonate

Heated to above 200 °C can be decomposed properly handle the waste gas.

3.14 Sodium pentachlorophenol

At 600 °C ~ 900 °C burning, and properly handle the waste gas.

3.15 Cumene hydroperoxide

Rotary kiln incineration, the temperature range of 820 °C ~ 1600 °C, burning a few seconds to properly handle the waste gas.

3.16 Daunorubicin

Rotary kiln incineration, the temperature range of 820 °C ~ 1600 °C, liquid incineration seconds, burning solid hours required to properly handle the waste gas.

Appendix A

Mutagens, teratogens and carcinogens in control English name, CAS and abbreviation Mutagens, teratogens and carcinogens in control English name, CAS and abbreviation in Table A.1.

Table A.1 mutagens, teratogens and carcinogens in control English name, CAS and abbreviation

Chinese name English name CAS abbreviation 2-acetamido-fluorene 2-acetamidofluorene 53-96-3 2-AAF Aflatoxin B1 aflatoxinB1 1162-65-8 AFB1 Benzo [a] anthracene, benz [a] anthracene 56-55-3 BA Benzidine 92-87-5 Bz Benzo [a] pyrene, benzo [a] pyrene 50-32-8 BP Cyclophosphamide 50-18-0 CP Amino-2,7-fluorene 2,7-diaminofluorene 525-64-4 2,7-AF 7,12-dimethyl-benz [a] anthracene-7,12-dimethylbenz [a] anthracene 57-97-6 DMBA Ethyl methane sulfonate ethylmethanesulphonate 62-50-0 EMS 3-methyl-MCA 3-methylcholanthrene 56-49-5 3-MC Methyl methanesulfonate 66-27-3 MMS Mitomycin C mitomycinC 50-07-7 MMC beta- naphthylamine 2-naphthylamine 91-59-8 2-NAP Ethylnitrosourea N-nitroso-N-ethylurea 759-73-9 ENU N- methyl -N- -N- nitro-nitrosoguanidine N-methyl-N'-nitro-N-nitrosoguanidine 70-25-7 MNNG N- nitroso-methyl urea N-nitroso-N-methylurea 684-93-5 MNU 2-amino-fluorene 2-aminofluorene 153-78-6 --N- 4- nitroquinoline-oxide 4-nitroquinoline-N-oxide 56-57-5 -N- NDMA N-nitrosodimethylamine 62-75-9 NDMA Ochratoxin A ochratoxinA 303-47-9 OA Sodium azide 26628-22-8 -ICR-170 ICR-170 146-59-8 -ICR-191 ICR-191 17070-45-0 -Triethylene melamine 51-18-3 -Furanose amide furylfuramide 3688-53-7 AF-2 9-amino acridine 9-aminoacridine 90-45-9 -2-nitro-fluoren-2-nitrofluorene 607-57-8 -Anthracene-9,10-dimethyl-9,10-dimethylanthracene 781-43-1 -Trinitro fluorenone, 2,4,7-trinitro-9H-fluoren-9-one 129-79-3 -

Table A.1 (CONTINUED)

Chinese name English name CAS abbreviation Congo Red 573-58-0 -Dimethylaminobenzaldehyde diazonium sulfonate p ara-dimethylaminobenzenediazo sodiumsulfonate 140-56-7--Sodium pentachlorophenol sodiumpentachlorophenolate 131-52-2 -Cumene hydroperoxide 80-15-9 -Daunorubicin 20830-81-3 -PCBs polychorinatedbiphenyls 1336-36-3 -