GA/T 2091.3-2023Forensic sciences - Reagents and consumables for capillary electrophoresis - Part 3: Running buffer (English PDF)

Foreword

This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1: Structure and Drafting Rules of Standardization Documents" Drafting.

This document is Part 3 of GA/T 2091 "Forensic Science Capillary Electrophoresis Reagents and Consumables". GA/T 2091 has been released with Lower part.

---Part 1: Separating gel;

---Part 2: Deionized formamide;

---Part 3: Buffer.

Please note that some content in this document may be subject to patents. The publisher of this document assumes no responsibility for identifying patents.

Introduction

Capillary electrophoresis reagent consumables are special reagent consumables used for forensic science DNA electrophoresis detection. They are used in conjunction with special genetic analyzers.

It plays a vital role in the accurate typing of DNA fragments such as STR loci, including various products such as separation gel, formamide and buffer.

Taste. In order to ensure that the basis used in the development, production, and testing of related products is practical, feasible, and standardized, a series of standards have been formulated. GA/T 2091 "Forensic Science Capillary Electrophoresis Reagents and Consumables" is planned to consist of three parts.

---Part 1: Separating gel. The purpose is to clarify the technical requirements of separation gels used in capillary electrophoresis for DNA testing in forensic science.

requirements, stipulating test methods, inspection rules, labeling, packaging, transportation and storage requirements.

---Part 2: Deionized formamide. The purpose is to clarify the deionized formaldehyde used in capillary electrophoresis for DNA testing in forensic science.

Technical requirements for amides, stipulating test methods, inspection rules, labeling, packaging, transportation and storage requirements.

---Part 3: Buffer. The purpose is to clarify the technical requirements of buffers used in capillary electrophoresis for DNA testing in forensic science.

requirements, stipulating test methods, inspection rules, labeling, packaging, transportation and storage requirements.

Forensic Science Capillary Electrophoresis Reagents and Consumables Part 3: Buffer

1 Scope

This document specifies the technical requirements, test methods, and inspection rules for buffers used in capillary electrophoresis for forensic science DNA testing.

Labeling, packaging, transportation and storage.

This document applies to buffers used in capillary electrophoresis for forensic DNA testing. Reference book for buffers used in other fields document.

2 Normative reference documents

The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, the dated quotations for undated referenced documents, only the version corresponding to that date applies to this document; for undated referenced documents, the latest version (including all amendments) applies to this document.

GB/T 37226-2018 Basic quality requirements for human fluorescently labeled STR composite amplification detection reagents for forensic science GA/T 382-2014 Forensic Science DNA Laboratory Construction Specifications GA/T 383-2014 Forensic Science DNA Laboratory Testing Specifications GA/T 1163-2014 Analysis and application of human DNA fluorescently labeled STR typing results HG/T 3921 Chemical reagent sampling and acceptance rules

3 Terms and definitions

The following terms and definitions apply to this document.

runningbuffer

It can provide a stable pH value and ionic strength solution for capillary electrophoresis, and maintain the pH value at both poles of the electrophoresis to only change within a certain range.

ionization and maintaining the ionic strength required for the electrophoresis process, thereby making the electrophoresis results stable.

electroosmotic flowelectroosmoticflow in capillary electrophoresis, under the action of an external electric field, the cations in the electric double layer on the inner wall of the capillary move toward the cathode, driving the capillary at the same time.

A fluid flow formed by the overall directional migration of solutes in a fluid.

4 Technical requirements